Université Clermont Auvergne, CNRS, Sigma Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.
University "Vasile Alecsandri" of Bacău, Faculty of Engineering, Chemical and Food Engineering Department, Bacău, Romania.
Bioresour Technol. 2020 Aug;310:123412. doi: 10.1016/j.biortech.2020.123412. Epub 2020 Apr 18.
The aim of this work was to demonstrate the feasibility of second-generation bioethanol production using for the first time a sawmill mixed feedstock comprising four softwood species, representative of biomass resource in Auvergne-Rhône-Alpes region (France). The feedstock was subjected to a microwave-assisted water/ethanol Organosolv pretreatment. The investigation focused on enzymatic hydrolysis of this pretreated sawmill feedstock (PSF) using Cellic® Ctec2 as the enzyme, followed by fermentation of the resulting sugar solution using Saccharomyces cerevisiae strain. The cellulose-rich PSF with 71% w/w cellulose content presented high saccharification yields (up to 80%), which made it perfect for subsequent fermentation; this yield was predicted vs. time up to 5.2% w/v PSF loading using a mathematical model fitted only on data at 1.5%. Finally, high PSF loading (7.5%) and scaleup were shown to impair the saccharification yield, but alcoholic fermentation could still be carried out up to 80% of the theoretical glucose-to-ethanol conversion yield.
本工作旨在首次展示使用包含四种软木物种的锯木厂混合原料生产第二代生物乙醇的可行性，这些物种代表了法国奥弗涅-罗讷-阿尔卑斯地区的生物质资源。该原料经过微波辅助水/乙醇有机溶剂预处理。研究集中在使用 Cellic® Ctec2 作为酶对预处理的锯木厂原料（PSF）进行酶解，然后使用酿酒酵母菌株对所得糖溶液进行发酵。纤维素含量丰富的 PSF 纤维素含量高达 71%，w/w，具有很高的糖化产率（高达 80%），非常适合后续发酵；这一产率是根据仅在 1.5%下的数据拟合的数学模型预测的，时间长达 5.2% w/v PSF 负载。最后，高 PSF 负载（7.5%）和放大规模被证明会降低糖化产率，但酒精发酵仍可进行，达到理论葡萄糖到乙醇转化率的 80%。
