Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa; Department of Veterinary Anatomy, University of West Kordofan, West Kordofan State, P.O Box 12942, Gebaish, Sudan.
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa.
Anim Reprod Sci. 2021 Apr;227:106733. doi: 10.1016/j.anireprosci.2021.106733. Epub 2021 Mar 9.
Di-n-butyl phthalate (DBP) is widely used as a plasticizer in personal care and medical products and is known to induce toxicity in the male reproductive organs in both mammals and birds. In this study, there was investigation of the effects of DBP on the epithelium of the rete testis, proximal, and distal efferent ductules and epididymal duct of adult Japanese quail (Coturnix japonica) following treatment with varying doses during the pre-pubertal and peri-pubertal periods. Pre-pubertal quail (n = 25) 4 weeks post-hatching were dosed orally with 10, 50, 200 and 400 mg DBP/kg/d, for 30 days and control birds were administered corn-oil only (n = 5 per group). Histo-metrically, there was lesser (P < 0.001) epithelial heights of the rete testis and efferent ductules in all quail DBP-treated groups, but not in the epididymal duct epithelium. There were no morphological change effects as a result of DBP treatments in the rete testis epithelium, while there were epithelial cytoplasmic vacuoles detected in the distal efferent ductule and epididymal duct of birds treated with 50, 200 and 400 mg DPB/kg/d. There were several lesions, including degenerative changes, cytoplasmic vacuoles, apoptosis and autophagy in the epithelium of the proximal efferent ductule in quail treated with 200 and 400 mg DBP/kg/d. Overall, the results indicate that treatment with DBP during the pre-pubertal period induced dose-dependent histometric and morphological changes in the epithelium of the epididymal region. It is concluded that the proximal efferent ductule was a highly sensitive component of the epididymal tissues of Japanese quail following treatment with DBP during the pre-pubertal period.
邻苯二甲酸二丁酯（DBP）广泛用作个人护理和医疗产品中的增塑剂，已知在哺乳动物和鸟类的雄性生殖器官中具有诱导毒性的作用。在这项研究中，研究了在青春期前和青春期期间以不同剂量处理时，DBP 对成年日本鹌鹑（Coturnix japonica）的睾丸网、近端和远端输出小管以及附睾管的上皮的影响。孵化后 4 周的青春期前鹌鹑（n=25）经口给予 10、50、200 和 400mg DBP/kg/d，持续 30 天，对照组仅给予玉米油（每组 5 只）。组织学上，所有 DBP 处理组的睾丸网和输出小管上皮高度均降低（P<0.001），但附睾管上皮高度不受影响。DBP 处理对睾丸网上皮没有形态改变作用，但在 50、200 和 400mg DBP/kg/d 处理的鸟类的远端输出小管和附睾管中检测到上皮细胞质空泡。在接受 200 和 400mg DBP/kg/d 处理的鸟类的近端输出小管上皮中，有几种病变，包括退行性变化、细胞质空泡、凋亡和自噬。总之，结果表明，在青春期前期间用 DBP 处理会导致附睾区域上皮的剂量依赖性组织学和形态学变化。结论是，在青春期前期间用 DBP 处理后，近端输出小管是日本鹌鹑附睾组织的一个高度敏感的组成部分。
