Zakariah Musa, Molele Reneilwe A, Mahdy Mohammed A A, Ibrahim Mohammed I A, McGaw Lyndy J
Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa; Department of Veterinary Anatomy, Faculty of Veterinary Medicine, PMB 1069 University of Maiduguri, Maiduguri, Nigeria.
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa.
Micron. 2022 Jan;152:103163. doi: 10.1016/j.micron.2021.103163. Epub 2021 Oct 21.
Exposure to dibutyl phthalate (DBP) induces testicular damage in mammals. However, studies on the effects of DBP on spermatogenic cells in birds are grossly lacking. Therefore, this study was designed to determine the effects of the pre-pubertal exposure to DBP on the histology and ultrastructure of spermatogenic cells in the testis of adult Japanese quail (Coturnix coturnix japonica). The birds were randomly divided into five dosage groups at the age of 4 weeks. The control group received a corn oil vehicle only (a dose of 1 mL/kg body weight), while the other four experimental groups received a daily dosage of 10, 50, 200, 400 mg/kg body weight of DBP (dissolved in corn oil), respectively with the aid of gastric lavage, for 30 days. Testicular samples were processed and examined by light microscopy and transmission electron microscopy. Histopathological evaluation revealed vacuole formation, germ cell degenerations, and the absence of spermatogenic cell series. Ultrastructurally, chromatin clumps in spermatocyte and degenerated spermatogonia with ruptured nuclear membranes resting on the distorted basement membranes were observed. Others were intracytoplasmic vacuoles in round spermatids and fragments of dense apoptotic bodies. In conclusion, the findings of the present study reveal that spermatogenic cells of Japanese quails seem to be more sensitive to DBP-induced degeneration compared to mammalian species studied. The Japanese quail could be used to monitor environmental contamination with low doses of DBP.
接触邻苯二甲酸二丁酯（DBP）会导致哺乳动物睾丸损伤。然而，关于DBP对鸟类生精细胞影响的研究严重缺乏。因此，本研究旨在确定青春期前接触DBP对成年日本鹌鹑（Coturnix coturnix japonica）睾丸中生精细胞的组织学和超微结构的影响。这些鸟在4周龄时被随机分为五个剂量组。对照组仅接受玉米油载体（剂量为1 mL/kg体重），而其他四个实验组分别通过灌胃接受每日剂量为10、50、200、400 mg/kg体重的DBP（溶于玉米油），持续30天。对睾丸样本进行处理，并通过光学显微镜和透射电子显微镜进行检查。组织病理学评估显示有空泡形成、生殖细胞变性以及生精细胞系列缺失。在超微结构上，观察到精母细胞中的染色质团块以及退化的精原细胞，其核膜破裂，位于扭曲的基底膜上。其他的是圆形精子细胞中的胞质内空泡和密集凋亡小体的碎片。总之，本研究结果表明，与所研究的哺乳动物物种相比，日本鹌鹑的生精细胞似乎对DBP诱导的变性更敏感。日本鹌鹑可用于监测低剂量DBP对环境的污染。
