Yao Miaomiao, Ge Wanying, Zhou Qian, Zhou Xin, Luo Manli, Zhao Yingbo, Wei Baodong, Ji Shujuan
College of Food Science, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang City 110866, PR China.
College of Food Science, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang City 110866, PR China.
Food Chem. 2021 Aug 1;352:129458. doi: 10.1016/j.foodchem.2021.129458. Epub 2021 Mar 3.
We investigated the effect of exogenous glutathione (GSH) on chilling injury (CI) in postharvest bell pepper fruits stored at low temperature and explored the mechanism of this treatment from the perspective of the ascorbate-glutathione (AsA-GSH) cycle. Compared with the control, fruits treated with exogenous GSH before refrigeration displayed only slight CI symptoms and mitigated CI-induced cell damage after 10 d. Moreover, the treated peppers had lower lipid peroxidation product, HO, and O content than those did the control. Glutathione treatment enhanced the ascorbate-glutathione cycle by upregulating CaAPX1, CaGR2, CaMDHAR1, and CaDHAR1 and the antioxidant enzymes APX, GR, and MDHAR associated with the ascorbate-glutathione cycle. Glutathione treatment also increased ascorbate and glutathione concentrations. Taken together, our results showed that exogenous GSH treatment could alleviate CI in pepper fruits during cold storage by triggering the AsA-GSH cycle and improving antioxidant capacity.
我们研究了外源谷胱甘肽（GSH）对低温贮藏的采后甜椒果实冷害（CI）的影响，并从抗坏血酸-谷胱甘肽（AsA-GSH）循环的角度探讨了这种处理的机制。与对照相比，冷藏前用外源GSH处理的果实仅表现出轻微的冷害症状，且在10天后减轻了冷害诱导的细胞损伤。此外，处理后的甜椒比对照具有更低的脂质过氧化产物、HO和O含量。谷胱甘肽处理通过上调CaAPX1、CaGR2、CaMDHAR1和CaDHAR1以及与抗坏血酸-谷胱甘肽循环相关的抗氧化酶APX、GR和MDHAR来增强抗坏血酸-谷胱甘肽循环。谷胱甘肽处理还增加了抗坏血酸和谷胱甘肽的浓度。综上所述，我们的结果表明，外源GSH处理可以通过触发AsA-GSH循环和提高抗氧化能力来减轻甜椒果实在冷藏期间的冷害。
