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加权基因共表达网络分析（WGCNA）揭示了扁穗牛鞭草根系中响应涝渍胁迫的枢纽基因。

WGCNA analysis reveals hub genes in the Hemarthria compressa roots in response to waterlogging stress.

作者信息

Li Wenwen, Zhou Xiaoli, Qu Minghao, Zheng Yuqian, Shen Bingna, Zeng Bing, Feng Yanlong, Pang Kaiyue, Wu Jiahai, Zeng Bing

机构信息

College of Animal Science and Technology, Southwest Un Iversity, Chongqing, China.

Institute of Grass-Fed Livestock, Chongqing Academy of Animal Sciences, Chongqing, China.

出版信息

Sci Rep. 2025 Apr 22;15(1):13841. doi: 10.1038/s41598-025-94873-7.

DOI:10.1038/s41598-025-94873-7
PMID:40263479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12015520/
Abstract

Hemarthria compressa is a high-quality forage resource in China. In recent years, waterlogging has frequently occurred, adversely affecting the growth and development of H. compressa. In order to investigate the physiological and molecular response mechanisms of H. compressa under waterlogging stress and identify hub genes involved in waterlogging tolerance, H. compressa roots from the GY (waterlogging-tolerant) and N1291 (waterlogging-sensitive) cultivars were selected as experimental materials in this study. The physiological indexes of H. compressa were measured, and transcriptome sequencing was carried out after 8 h and 24 h of waterlogging stress, with 0 h used as the control group. Superoxide dismutase (SOD) and peroxidase (POD) activities were significantly increased in both GY and N1291 under waterlogging stress (P < 0.05). Weighted gene co-expression network analysis (WGCNA) identified a total of four modules significantly associated with waterlogging stress (r>|0.9|, P < 0.05). Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment results showed that differentially expressed genes (DEGs) were mainly enriched in the Starch and sucrose metabolism, Plant hormone signal transduction, Ribosome and Glutathione metabolism pathways. Seven hub genes were also retrieved, including Cluster-38255.67514 and Cluster-38255.80127, potentially associated with waterlogging tolerance. It is related to the Ribosome pathway and participates in the process of anti-waterlogging regulation. The results of this experiment provide new insights into the response mechanisms of H. compressa to waterlogging stress and a theoretical framework for the effective selection and breeding of waterlogging-tolerant cultivars.

摘要

扁穗牛鞭草是中国一种优质的饲料资源。近年来，涝害频繁发生，对扁穗牛鞭草的生长发育产生不利影响。为了研究扁穗牛鞭草在涝渍胁迫下的生理和分子响应机制，并鉴定参与耐涝性的关键基因，本研究选取了耐涝品种GY和涝敏感品种N1291的扁穗牛鞭草根系作为实验材料。测定了扁穗牛鞭草的生理指标，并在涝渍胁迫8小时和24小时后进行转录组测序，以0小时作为对照组。在涝渍胁迫下，GY和N1291的超氧化物歧化酶（SOD）和过氧化物酶（POD）活性均显著增加（P < 0.05）。加权基因共表达网络分析（WGCNA）共鉴定出四个与涝渍胁迫显著相关的模块（r > |0.9|，P < 0.05）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集结果表明，差异表达基因（DEGs）主要富集在淀粉和蔗糖代谢、植物激素信号转导、核糖体和谷胱甘肽代谢途径中。还检索到7个关键基因，包括Cluster-38255.67514和Cluster-38255.80127，可能与耐涝性有关。它与核糖体途径相关，参与抗涝调节过程。本实验结果为扁穗牛鞭草对涝渍胁迫的响应机制提供了新的见解，并为耐涝品种的有效选育提供了理论框架。

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