壳寡糖乳酸盐包裹的超小氧化钆纳米颗粒的合成、细胞毒性和弛豫性能。

Chitosan Oligosaccharide Lactate-Coated Ultrasmall Gadolinium Oxide Nanoparticles: Synthesis, Cytotoxicity, and Relaxometric Properties.

机构信息

Department of Chemistry, College of Natural Sciences, Kyungpook National University, Taegu 41566, South Korea.

Department of Molecular Medicine, School of Medicine, Kyungpook National University, Taegu 41944, South Korea.

出版信息

J Nanosci Nanotechnol. 2021 Aug 1;21(8):4145-4150. doi: 10.1166/jnn.2021.19378.

DOI:10.1166/jnn.2021.19378

PMID:33714294

Abstract

In this study, hydrophilic and biocompatible chitosan oligosaccharide lactate (COL)-coated ultra-small gadolinium oxide nanoparticles (NPs) were synthesized through a one-pot polyol method and characterized by various experimental techniques. The cellular cytotoxicity assay indicated that the COL-coated gadolinium oxide NPs were non-toxic up to 500 M Gd. In addition, their water proton spin relaxivities (i.e., and ₂) were estimated to be 13.0 and 27.0 smM, respectively, which are higher than those of commercial magnetic resonance imaging (MRI) contrast agents. The application potential of the solution sample as a T MRI contrast agent was demonstrated by measuring map images in which dose-dependent contrast enhancements were observed.

摘要

在这项研究中，通过一步多元醇法合成了亲水性和生物相容性的壳聚糖低聚糖乳酸盐（COL）包覆的超小氧化钆纳米颗粒（NPs），并通过各种实验技术对其进行了表征。细胞毒性测定表明，壳聚糖包覆的氧化钆纳米颗粒在高达 500 M Gd 的浓度下是无毒的。此外，它们的水质子自旋弛豫率（即和 ₂）分别估计为 13.0 和 27.0 smM，高于商业磁共振成像（MRI）对比剂。通过测量图谱图像证明了溶液样品作为 T₁ MRI 对比剂的应用潜力，其中观察到了剂量依赖性的对比增强。