Raeeszadeh Mahdieh, Rezaee Mahtab, Akbari Abolfazl, Khademi Nadia
Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Graduate of Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Drug Chem Toxicol. 2022 Sep;45(5):2031-2038. doi: 10.1080/01480545.2021.1895826. Epub 2021 Mar 10.
The gentamicin renal toxicity has been the focal point for much discussion. The objective of this study was to investigate the impacts of extract and vitamin C on gentamicin dose-dependent toxicity in rats' kidney. The present study was conducted on 60 male Wistar rats divided into ten experimental groups: control (untreated), G1, G2, G3 (100, 200, 300 mg/kg gentamicin), M1, M2 and M3 (500 mg/kg marjoram extract) + 100, 200 and 300 gentamicin, V1, V2 and V3 (Vitamin C 500 mg/kg) + 100, 200 and 300 of gentamicin. On the last day, the serum was separated from heart blood and the kidney tissues were extracted to measure the biochemical and oxidative stress parameters and histological changes. Kidney damage was confirmed as dose-dependent gentamicin by biochemical and pathological parameters. Urea, Blood Urea Nitrogen (BUN), and creatinine showed a significant increase in the G3 group compared to the control, M1, and V1 groups ( < 0.01). Catalase (CAT), Glutathione peroxidase (GPx), and Superoxide dismutase (SOD) showed a significant reduction in renal tissue in the G3 group compared to the other groups ( < 0.001). Malondialdehyde (MDA) concentration in the kidney tissue of the G3 group also showed a significant increase compared to other groups ( < 0.001). Furthermore, TNFα and IL-1 levels were the highest in the G3 group, and serum total antioxidant capacity (TAC) concentration had the lowest amount compared to other groups. Moreover, histopathological lesions of the kidney showed significant statistical differences among the groups that received gentamicin with the control and M1 group. Marjoram extract at the dose of 500 mg/kg had a desirable effect on controlling gentamicin damage in the kidneys compared with vitamin C. In particular, controlling gentamicin-induced oxidative stress and inflammation by the consumption of marjoram extract and vitamin C plays an important role in protecting the kidneys.
庆大霉素肾毒性一直是诸多讨论的焦点。本研究的目的是调查牛至提取物和维生素C对大鼠肾脏中庆大霉素剂量依赖性毒性的影响。本研究选用60只雄性Wistar大鼠，分为10个实验组：对照组（未处理）、G1、G2、G3（100、200、300 mg/kg庆大霉素）、M1、M2和M3（500 mg/kg牛至提取物）+100、200和300庆大霉素、V1、V2和V3（500 mg/kg维生素C）+100、200和300庆大霉素。在最后一天，从心脏血液中分离血清，并提取肾脏组织以测量生化和氧化应激参数以及组织学变化。通过生化和病理参数证实肾脏损伤为庆大霉素剂量依赖性。与对照组、M1组和V1组相比，G3组的尿素、血尿素氮（BUN）和肌酐显著升高（<0.01）。与其他组相比，G3组肾组织中的过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）显著降低（<0.001）。与其他组相比，G3组肾组织中的丙二醛（MDA）浓度也显著升高（<0.001）。此外，G3组的TNFα和IL-1水平最高，血清总抗氧化能力（TAC）浓度与其他组相比最低。此外，接受庆大霉素的组与对照组和M1组之间肾脏的组织病理学损伤存在显著统计学差异。与维生素C相比，500 mg/kg剂量的牛至提取物对控制肾脏中的庆大霉素损伤具有理想效果。特别是，通过食用牛至提取物和维生素C来控制庆大霉素诱导的氧化应激和炎症在保护肾脏方面发挥着重要作用。
