Department of Biosciences, Mokpo National University, Muan 58554, Republic of Korea.
J Nanosci Nanotechnol. 2021 Jul 1;21(7):3673-3678. doi: 10.1166/jnn.2021.19159.
Infectious salmon anemia virus (ISAV) is an orthomyxovirus causing fetal disease of farmed Atlantic salmon, leading to considerable financial losses farmers around the world. In the present study, we sequenced and identified microRNAs (miRNAs) from Atlantic salmon kidney (ASK) cells infected with ISAV. Based on initial experimental data derived from RNA-Seq analysis, a group of differentially expressed (DE) miRNAs from the infected ASK cells were selected for expression analysis and to identify their mRNA targets. Among the DE miRNAs, highest-ranked 19 up-or down-regulated miRNAs stood out as attractive candidates for a role in ISAV-related function, which displayed a clear tendency to be continuously upregulated or downregulated during viral infection. Interestingly, these miRNAs displayed significant relationships with immune system processes based on their mRNA targets. Besides, miR-148a/b and miR-152 can be putative anti-viral miRNAs by directly targeting viral genes such as HA, P3, and NP genes which are required for viral infection. Taken together, these data may provide new clues to understanding the molecular framework of immune defense response during viral infection.
传染性鲑鱼贫血病毒（ISAV）是一种正粘病毒，可引起养殖大西洋鲑的胎儿疾病，给世界各地的农民造成了巨大的经济损失。在本研究中，我们对感染 ISAV 的大西洋鲑肾（ASK）细胞中的 microRNAs（miRNAs）进行了测序和鉴定。基于来自 RNA-Seq 分析的初步实验数据，选择了一组差异表达（DE）的 miRNA 进行表达分析，并鉴定其 mRNA 靶标。在 DE miRNAs 中，排名最高的 19 个上调或下调的 miRNA 作为与 ISAV 相关功能相关的候选物脱颖而出，它们在病毒感染过程中表现出明显的持续上调或下调趋势。有趣的是，这些 miRNA 基于其 mRNA 靶标与免疫系统过程显示出显著的关系。此外，miR-148a/b 和 miR-152 可以通过直接靶向病毒基因（如 HA、P3 和 NP 基因）作为推定的抗病毒 miRNA，这些基因是病毒感染所必需的。总之，这些数据可能为理解病毒感染期间免疫防御反应的分子框架提供新的线索。
