Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Microb Pathog. 2018 Oct;123:353-360. doi: 10.1016/j.micpath.2018.07.023. Epub 2018 Jul 21.
Infectious salmon anemia virus (ISAV) is the causative agent of infectious salmon anemia (ISA), a relatively novel disease primarily affecting farmed salmon species, primarily in Salmo salar specimens, causing severe outbreaks in most producer countries. Although ISAV has been extensively studied at the molecular level, not much is known about the host/cell interaction at the small RNA level. MicroRNAs (miRNAs) are small, non-coding RNA that regulate mRNA expression at the post-transcriptional level. In recent years, the putative role of these molecules in host-pathogen interactions has drawn particular attention because of their pivotal involvement as regulatory elements in a number of eukaryotic organisms. Given the importance of the salmon industry in Chile, a deep understanding of the interaction between ISAV and its hosts is of importance. In the present work, we studied the kinetic expression of selected miRNAs during ISAV infection, both in vitro and in vivo. Based on initial experimental data derived from a small RNA-Seq analysis, a group of miRNAs that were differentially expressed in infected cells were selected for analysis. As a result, two miRNAs, miR-462a-5p and miR-125 b-5p, showed increased and decreased expression, respectively, during ISAV infection.
传染性鲑鱼贫血病毒（ISAV）是传染性鲑鱼贫血症（ISA）的病原体，ISA 是一种相对较新的疾病，主要影响养殖鲑鱼物种，主要是在 Salmo salar 标本中，在大多数生产国中引起严重疫情。尽管 ISAV 在分子水平上已经得到了广泛的研究，但对于小 RNA 水平上的宿主/细胞相互作用知之甚少。MicroRNAs (miRNAs) 是一种小型非编码 RNA，可在转录后水平调节 mRNA 的表达。近年来，由于这些分子在许多真核生物中作为调节因子的关键作用，它们在宿主-病原体相互作用中的潜在作用引起了特别关注。鉴于智利鲑鱼产业的重要性，深入了解 ISAV 与其宿主之间的相互作用非常重要。在本工作中，我们研究了 ISAV 感染过程中选定 miRNA 的动态表达，包括体外和体内。基于从小 RNA-Seq 分析中获得的初始实验数据，选择了一组在感染细胞中差异表达的 miRNA 进行分析。结果表明，在 ISAV 感染过程中，miR-462a-5p 和 miR-125b-5p 两种 miRNA 的表达分别增加和减少。
