Department of Genetics, Faculty of advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Medical Genetics, Cancer Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gulf J Oncolog. 2021 Jan;1(35):7-13.
Lung cancer is the most deadly and sumptuous cancer across the globe. Cancer occurrence is increasing progressively and there is no ideal cure yet. Therefore, new therapeutic areas are needed. The use of herbal extracts due to its properties such as antioxidant activity, anti-proliferative effect, and few side effects can be promising in the treatment of cancer. This study aimed to compare the effect of Echinophora platyloba DC. and Cordia myxa L extracts on apoptosis induction in A549 cancer cells.
In this experiment, the A549 cell line was first cultured in DMEM medium containing 10% FBS and then treated with different concentrations of both compounds. MTT assay was performed to determine IC50 and to compare the viability of cells treated with different concentrations of Echinophora platyloba DC. and Cordia myxa L seed on days 1, 3 and 5. QRT-PCR test was used to investigate the effects of Echinophora platyloba DC. and Cordia myxa L with IC50 on apoptosis induction.
MTT results showed that both plant extracts resulted in cell death and decreased viability of lung cancer cells. But the percentage of viability decreased by Echinophora platyloba DC. was more. Also, Echinophora platyloba DC. significantly increased the expression of Bax, P53 and Bad apoptotic genes and decreased the expression of Bcl2 gene, which induces apoptotic death and the cytotoxic effect of Echinophora platyloba DC. over Cordia myxa L.
In comparing the effects of these two extracts Echinophora platyloba DC. was more effective than Cordia myxa L and had greater cytotoxicity on A549 cancerous cells in a lesser concentration and could be an appropriate drug candidate for the treatment of lung cancer.
肺癌是全球最致命和最昂贵的癌症。癌症的发病率正在逐渐上升，但目前仍没有理想的治疗方法。因此，需要新的治疗领域。由于具有抗氧化活性、抗增殖作用和副作用少等特性，草药提取物在癌症治疗中可能具有广阔的前景。本研究旨在比较沙蓬和蓝桉提取物对 A549 癌细胞凋亡诱导的影响。
在这项实验中，首先将 A549 细胞系在含有 10% FBS 的 DMEM 培养基中培养，然后用不同浓度的两种化合物处理。通过 MTT 测定法确定 IC50，并比较不同浓度的沙蓬和蓝桉种子处理对细胞活力的影响，在第 1、3 和 5 天进行。QRT-PCR 试验用于研究沙蓬和蓝桉与 IC50 对凋亡诱导的影响。
MTT 结果表明，两种植物提取物均导致细胞死亡并降低肺癌细胞的活力。但沙蓬提取物的细胞活力下降幅度更大。此外，沙蓬提取物显著增加了 Bax、P53 和 Bad 凋亡基因的表达，降低了 Bcl2 基因的表达，从而诱导细胞凋亡和沙蓬提取物的细胞毒性作用超过蓝桉。
在比较这两种提取物的作用时，沙蓬比蓝桉更有效，在较低浓度下对 A549 癌细胞具有更强的细胞毒性，可能是治疗肺癌的合适候选药物。
