Department of Highly Advanced Stomatology, Graduate School of Dentistry, Kanagawa Dental University.
Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University.
Biocontrol Sci. 2021;26(1):17-25. doi: 10.4265/bio.26.17.
Dental materials are inevitably contaminated with oral microorganisms. To prevent transmission of infectious diseases, impressions need to be disinfected. In the present study, we examined the disinfection effects on impression materials and biofilm removal by sodium dichloroisocyanurate (SDIC). Exponentially growing Streptococcus mutans, Escherichia coli, Staphylococcus aureus and Candida albicans, and dental plaque bacteria were suspended in phosphate buffered saline (PBS) and exposed for 1, 5 and 10 min to 1 mL of the 10 ppm, 100 ppm, 1,000 ppm, and 10,000 ppm SDIC solutions. The bactericidal effect was evaluated by colony forming units of each microorganisms. Moreover, the effect of SDIC solution on S. mutans biofilm was examined. Bactericidal effects of SDIC solutions on oral bacteria on dental impression surfaces were assessed and the surface quality of dental casts after immersion in SDIC solution for 30 min was observed under a scanning electron microscope. The number of all bacterial strains, including plaque bacteria, were significantly decreased by SDIC solution treatment in a dose-dependent manner. Significant S. mutans biofilm removing activity of SDIC was observed in 1,000 and 10,000 ppm solution. The number of oral bacteria adhering to the surfaces of impressions markedly decreased following 10-min immersion in the 1,000 ppm SDIC solution. The 30-min immersion of dental impression in the 1,000 ppm SDIC solution did not adversely affect the surface roughness of dental casts. The results indicate that SDIC Solution is useful to deactivate oral bacteria on dental impression.
牙科材料不可避免地会被口腔微生物污染。为了防止传染病的传播，印模需要进行消毒。在本研究中，我们研究了二氯异氰尿酸钠（SDIC）对印模材料的消毒效果和生物膜去除效果。指数生长期的变形链球菌、大肠杆菌、金黄色葡萄球菌和白色念珠菌以及牙菌斑细菌悬浮在磷酸盐缓冲液（PBS）中，并分别暴露于 10ppm、100ppm、1000ppm 和 10000ppm SDIC 溶液 1ml 中 1、5 和 10min。通过每个微生物的菌落形成单位评估杀菌效果。此外，还研究了 SDIC 溶液对 S. mutans 生物膜的影响。评估了 SDIC 溶液对牙科印模表面口腔细菌的杀菌效果，并在扫描电子显微镜下观察了 SDIC 溶液浸泡 30min 后牙科模型表面的质量。SDIC 溶液处理以剂量依赖的方式显著减少了所有细菌菌株（包括菌斑细菌）的数量。在 1000ppm 和 10000ppm 溶液中观察到 SDIC 对 S. mutans 生物膜具有显著的去除活性。在 1000ppm SDIC 溶液中浸泡 10min 后，印模表面附着的口腔细菌数量明显减少。将牙科印模浸入 1000ppm SDIC 溶液中 30min 不会对牙科模型的表面粗糙度产生不利影响。结果表明，SDIC 溶液可有效灭活牙科印模上的口腔细菌。
