Jabeen Bushra, Mirani Zulfiqar A, Lone Muneeb A, Nirkhiwale Arpit, Farooqui Waqas A, Aslam Kashif, Ahmed Muhammad Adeel, Vohra Fahim
Department of Prosthodontics, Dow International Dental College, Dow University of Health Sciences, Karachi, Pakistan.
Department of Microbiology, Pakistan Council of Scientific & Industrial Research Laboratories Complex, Karachi, Pakistan.
Eur J Dent. 2025 May;19(2):346-353. doi: 10.1055/s-0044-1788631. Epub 2024 Dec 10.
The present study evaluated the effect of chemical disinfectants and microwave sterilization on the removal of biofilm containing , , and from type IV dental stone.
One hundred twenty-eight ( = 128) type IV dental cast stone specimens were prepared, and biofilms of microorganisms were cultured. Dental stone samples were subjected to disinfection protocols, including 0.5% chlorhexidine (CHX), 0.5% sodium hypochlorite (NaOCl), 20% neem extract, and microwave irradiation for 1 to 5 minutes. Colony forming unit (CFU) counts and scanning electron microscopy were utilized to witness changes in the biofilm, pre- and postdisinfection/sterilization.
For , significant ( < 0.05) decrease in CFU counts after 1 minute (from 233 to -215) and 2, 3, and 5 minutes (from 233 to -233) were observed after CHX treatment. After microwave radiation, a significant decrease in CFU counts was also observed after 1 minute (from 233 to -130.3), 2 minutes (from 233 to -229), and 3 and 5 minutes (from 233 to -233). For , a significant ( < 0.05) decrease in CFU counts was observed after 1, 2, 3, and 5 minutes (from 212 to -268) after NaOCl treatment and microwave radiation (from 212 to -271 after 1 minute and from 212 to -274.3 after 2, 3, and 5 minutes). For , significant ( < 0.05) decrease in the CFU counts (1-5 minutes) was observed after CHX exposure, while NaOCl and microwave radiation demonstrated equal disinfection potency. Neem extract was effective to disinfect the dental stone; however, it was not as potent as the other disinfectants and microwave radiation.
It was observed that exposure to CHX, NaOCl, and microwave radiation significantly reduced the microbial CFU counts. Although the use of neem extract also significantly reduced these CFU counts, this reduction was not as much as the other three tested materials. Further research exploring other chemical disinfectants with various concentrations is recommended.
本研究评估了化学消毒剂和微波灭菌对从IV型牙科石膏中去除含 、 和 的生物膜的效果。
制备了128个IV型牙科铸型石膏标本，并培养了微生物生物膜。牙科石膏样本接受消毒方案处理，包括0.5%洗必泰（CHX）、0.5%次氯酸钠（NaOCl）、20%印楝提取物以及1至5分钟的微波照射。利用菌落形成单位（CFU）计数和扫描电子显微镜观察消毒/灭菌前后生物膜的变化。
对于 ，CHX处理后，1分钟（从233降至 -215）以及2、3和5分钟（从233降至 -233）后CFU计数显著（ ＜0.05）降低。微波辐射后，1分钟（从233降至 -130.3）、2分钟（从233降至 -229）以及3和5分钟（从233降至 -233）后CFU计数也显著降低。对于 ，NaOCl处理后1、2、3和5分钟（从212降至 -268）以及微波辐射后（1分钟后从212降至 -271，2、3和5分钟后从212降至 -274.3）CFU计数显著（ ＜0.05）降低。对于 ，CHX暴露后（1 - 5分钟）CFU计数显著（ ＜0.05）降低，而NaOCl和微波辐射显示出同等的消毒效力。印楝提取物对牙科石膏有消毒效果；然而，其效力不如其他消毒剂和微波辐射。
观察到暴露于CHX、NaOCl和微波辐射显著降低了微生物CFU计数。虽然使用印楝提取物也显著降低了这些CFU计数，但降低程度不如其他三种测试材料。建议进一步研究探索不同浓度的其他化学消毒剂。
