de la Luna S, Soria I, Pulido D, Ortín J, Jiménez A
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Gene. 1988;62(1):121-6. doi: 10.1016/0378-1119(88)90585-9.
Recombinant plasmids have been obtained that lead to the accumulation of five- to ten-fold more puromycin-N-acetyl-transferase (PAC) mRNA and two- to three-fold more PAC activity than the already described plasmid pSV2pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117-4124]. When these optimized recombinants were used for stable transformation to puromycin resistance, efficiencies up to 1 x 10(-2) were obtained, indicating that these pac-containing recombinants may be very useful dominant selectable markers for gene transfer in mammalian cells.
已获得重组质粒，其导致嘌呤霉素 - N - 乙酰转移酶（PAC）mRNA的积累比已描述的质粒pSV2pac多五至十倍，PAC活性多两至三倍[瓦拉等人，《核酸研究》14（1986）4117 - 4124]。当这些优化的重组体用于稳定转化以获得嘌呤霉素抗性时，获得了高达1×10⁻²的效率，这表明这些含pac的重组体可能是用于哺乳动物细胞基因转移的非常有用的显性选择标记。
