Buhr Trevor J, Reed Carter H, Shoeman Allyse, Bauer Ella E, Valentine Rudy J, Clark Peter J
Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, United States.
Neuroscience Program, Iowa State University, Ames, IA, United States.
Front Behav Neurosci. 2021 Feb 25;15:639790. doi: 10.3389/fnbeh.2021.639790. eCollection 2021.
Monoamine neurotransmitter activity in brain reward, limbic, and motor areas play key roles in the motivation to misuse alcohol and can become modified by exercise in a manner that may affect alcohol craving. This study investigated the influence of daily moderate physical activity on monoamine-related neurochemical concentrations across the mouse brain in response to high volume ethanol ingestion. Adult female C57BL/6J mice were housed with or without 2.5 h of daily access to running wheels for 30 days. On the last 5 days, mice participated in the voluntary binge-like ethanol drinking procedure, "Drinking in the dark" (DID). Mice were sampled immediately following the final episode of DID. Monoamine-related neurochemical concentrations were measured across brain regions comprising reward, limbic, and motor circuits using ultra High-Performance Liquid Chromatography (UHPLC). The results suggest that physical activity status did not influence ethanol ingestion during DID. Moreover, daily running wheel access only mildly influenced alcohol-related norepinephrine concentrations in the hypothalamus and prefrontal cortex, as well as serotonin turnover in the hippocampus. However, access to alcohol during DID eliminated wheel running-related decreases of norepinephrine, serotonin, and 5-HIAA content in the hypothalamus, but also to a lesser extent for norepinephrine in the hippocampus and caudal cortical areas. Finally, alcohol access increased serotonin and dopamine-related neurochemical turnover in the striatum and brainstem areas, regardless of physical activity status. Together, these data provide a relatively thorough assessment of monoamine-related neurochemical levels across the brain in response to voluntary binge-patterned ethanol drinking, but also adds to a growing body of research questioning the utility of moderate physical activity as an intervention to curb alcohol abuse.
大脑奖赏、边缘系统和运动区域中的单胺神经递质活性在酒精滥用动机中起关键作用，并且可能会因运动而发生改变，这种改变方式可能会影响对酒精的渴望。本研究调查了日常适度体育活动对小鼠大脑中与单胺相关的神经化学物质浓度的影响，这些小鼠因大量摄入乙醇而产生反应。成年雌性C57BL/6J小鼠被饲养30天，一组有每天2.5小时使用跑步轮的机会，另一组没有。在最后5天，小鼠参与了自愿性暴饮暴食式乙醇饮用程序，即“黑暗中饮酒”（DID）。在DID的最后一次发作后立即对小鼠进行采样。使用超高效液相色谱（UHPLC）测量包括奖赏、边缘系统和运动回路在内的大脑区域中与单胺相关的神经化学物质浓度。结果表明，体育活动状态在DID期间并未影响乙醇摄入。此外，每天使用跑步轮仅对下丘脑和前额叶皮质中与酒精相关的去甲肾上腺素浓度以及海马体中的5-羟色胺周转率有轻微影响。然而，在DID期间接触酒精消除了跑步轮使用相关的下丘脑去甲肾上腺素、5-羟色胺和5-羟吲哚乙酸含量的降低，但在海马体和尾侧皮质区域对去甲肾上腺素的影响程度较小。最后，无论体育活动状态如何，接触酒精都会增加纹状体和脑干区域中与5-羟色胺和多巴胺相关的神经化学物质周转率。总之，这些数据提供了对大脑中与单胺相关的神经化学物质水平的相对全面评估，以应对自愿性暴饮暴食模式的乙醇饮用，同时也增加了越来越多的研究，质疑适度体育活动作为遏制酒精滥用干预措施的效用。
