University of Colorado, Institute for Behavioral Genetics, 1480 30th Street, 447 University of Colorado at Boulder, Boulder, CO, 80309-0447, United States; Department of Integrative Physiology, 354 University of Colorado at Boulder, Boulder, CO, 80309-0354, United States.
University of Colorado, Institute for Behavioral Genetics, 1480 30th Street, 447 University of Colorado at Boulder, Boulder, CO, 80309-0447, United States; School of Mathematics, University of Minnesota, 206 Church Street SE, Minneapolis, MN, 55455, United States.
Alcohol. 2019 Jun;77:91-99. doi: 10.1016/j.alcohol.2018.10.007. Epub 2018 Oct 25.
The mesolimbic dopaminergic reward pathway is activated by both alcohol and exercise, suggesting exercise as a possible treatment or preventative method for alcohol-use disorders (AUDs). Prior studies conducted in our lab have demonstrated the hedonic substitution of voluntary alcohol consumption for voluntary wheel running in female C57Bl/6Ibg mice, and a trend in male C57Bl/6Ibg mice. Given the important contribution of genetic background on AUDs, this study aims to assess the effects of voluntary wheel running on voluntary alcohol consumption in two moderate alcohol-consuming strains of mice, C3H/Ibg and 129/SvEvTac. Contrary to our previous studies conducted in C57Bl/6Ibg mice, 129/SvEvTac and male C3H/Ibg mice housed without a wheel consumed significantly more alcohol than mice housed with a free or locked wheel. This suggests that 129/SvEvTac and male C3H/Ibg mice are reducing their alcohol consumption due to an enriched environment and not exercise. Interestingly, the three groups of female C3H/Ibg mice (free wheel, locked wheel, no wheel) did not significantly differ in alcohol consumption, suggesting sex-specific differences in C3H/Ibg mice. In addition, genetic and sex effects were observed for running phenotypes in the presence of alcohol. Female 129/SvEvTac and C57Bl/6Ibg mice ran longer distances than male mice, whereas male and female C3H/Ibg mice did not differ in distance run. C3H/Ibg and female 129/SvEvTav mice with access only to water ran longer distances than mice with access to both alcohol and water. However, this effect was not observed in C57Bl/6Ibg or male 129/SvEvTac mice. The results of this mouse model highlight the importance of genetic background and sex on an animal's response to exercise as an enrichment to reduce voluntary alcohol consumption.
中脑边缘多巴胺奖赏通路既被酒精激活，也被运动激活，这表明运动可能是治疗或预防酒精使用障碍（AUD）的一种方法。我们实验室之前的研究表明，在雌性 C57Bl/6Ibg 小鼠中，自愿饮酒会被自愿轮跑替代，而在雄性 C57Bl/6Ibg 小鼠中则出现了这种替代的趋势。鉴于遗传背景对 AUD 的重要贡献，本研究旨在评估自愿轮跑对两种中高酒精摄入小鼠（C3H/Ibg 和 129/SvEvTac）自愿饮酒的影响。与我们之前在 C57Bl/6Ibg 小鼠中进行的研究相反，129/SvEvTac 和雄性 C3H/Ibg 小鼠在没有轮子的情况下摄入的酒精量明显多于有自由轮或锁定轮的小鼠。这表明 129/SvEvTac 和雄性 C3H/Ibg 小鼠由于环境丰富而减少了饮酒量，而不是运动。有趣的是，三组雌性 C3H/Ibg 小鼠（自由轮、锁定轮、无轮）的饮酒量没有显著差异，这表明 C3H/Ibg 小鼠存在性别特异性差异。此外，在存在酒精的情况下，还观察到了遗传和性别对跑步表型的影响。雌性 129/SvEvTac 和 C57Bl/6Ibg 小鼠的跑动距离比雄性小鼠长，而雄性和雌性 C3H/Ibg 小鼠的跑动距离没有差异。只接触水的 C3H/Ibg 和雌性 129/SvEvTav 小鼠的跑动距离比同时接触水和酒精的小鼠长。然而，在 C57Bl/6Ibg 或雄性 129/SvEvTac 小鼠中没有观察到这种效果。本小鼠模型的结果强调了遗传背景和性别的重要性，它们会影响动物对运动的反应，运动是一种减少自愿饮酒的丰富方式。
