Cave Rory, Misra Raju, Chen Jiazhen, Wang Shiyong, Mkrtchyan Hermine V
School of Health, Sport and Bioscience, University of East London, London, United Kingdom.
Natural History Museum, Core Research Laboratories, Molecular Biology, London, United Kingdom.
Front Microbiol. 2021 Feb 25;12:576696. doi: 10.3389/fmicb.2021.576696. eCollection 2021.
Coagulase-negative staphylococci (CoNS) have been recovered from different ecological niches, however, little is known about the genetic relatedness of these isolates. In this study, we used whole genome sequencing to compare positive ( ) , and isolates recovered from hand-touched surfaces from general public settings in East and West London with data of isolates deposited to European Nucleotide Archive (ENA) by other research groups. These included isolates associated with hospital settings (including those recovered from patients), healthy humans, livestock, pets, plants and natural, and other public environments. Using core and accessory phylogenetic analyses we were able to identify that the and isolates recovered from general public settings were genetically related to isolates recovered from the bloodstream, urinary tract and eye infections. isolates recovered in our study were also shown to be genetically related to isolates previously recovered from livestock/livestock housing, whereas isolates were genetically related to isolates recovered from a dog and kefir (fermented cow milk drink). isolates were not genetically related to any isolates recovered from clinical samples but were genetically related to isolates recovered from mosquitoes, air samples (residential areas) and kefir. All three species showed to have genetic relatedness to isolates recovered from healthy humans. These results show that CoNS isolates in this study share genetic similarities with those of different lineages and that and isolates found in general public settings in this study may pose a risk to public health.
凝固酶阴性葡萄球菌（CoNS）已从不同的生态位中分离出来，然而，对于这些分离株的遗传相关性却知之甚少。在本研究中，我们使用全基因组测序技术，将从伦敦东部和西部公共场所的手触表面分离得到的阳性（）、和分离株，与其他研究小组存入欧洲核苷酸档案馆（ENA）的分离株数据进行比较。这些分离株包括与医院环境相关的（包括从患者身上分离得到的）、健康人、牲畜、宠物、植物以及自然和其他公共环境中的分离株。通过核心和辅助系统发育分析，我们能够确定从公共场所分离得到的和分离株在基因上与从血液、泌尿道和眼部感染中分离得到的菌株相关。我们研究中分离得到的分离株在基因上也与先前从牲畜/牲畜饲养场分离得到的菌株相关，而分离株在基因上与从狗和开菲尔（发酵牛奶饮品）中分离得到的菌株相关。分离株在基因上与从临床样本中分离得到的任何菌株均无关联，但与从蚊子、空气样本（居民区）和开菲尔中分离得到的菌株在基因上相关。所有这三个菌种均显示与从健康人身上分离得到的菌株具有遗传相关性。这些结果表明，本研究中的CoNS分离株与不同谱系的分离株具有遗传相似性，并且本研究在公共场所发现的和分离株可能对公众健康构成风险。
