Naz Hira, Naz Sidra, Miraj Rabab, Zaheer Akfish, Azam Nada, Mughal Isham Saleem, Khan Abdul Wali, Ishaq Muhammad, Sundas Fnu, Hanif Muhammad
Internal Medicine, Post Graduate Medical Institute, Lahore General Hospital, Lahore, PAK.
Internal Medicine, University of Health Sciences (UHS), Lahore, PAK.
Cureus. 2021 Feb 7;13(2):e13186. doi: 10.7759/cureus.13186.
Background The principal manifestation of hyperuricemia is gout. Many drugs are in use nowadays to treat gout, but they are linked with multiple side effects. The present study observed berberine (from Chinese folk medicine) on serum and urinary uric acid levels in rats with potassium oxonate-induced hyperuricemia. Materials and methods Thirty-six adult healthy female Sprague Dawley rats were randomly divided into six groups of six rats each. To induce hyperuricemia, all the groups except Group A were given potassium oxonate (250 mg/kg) intraperitoneally on days 1, 3, and 7. Group A, the normal control group, was given normal saline for seven consecutive days intraperitoneally. Group C was administered allopurinol (5 mg/kg body weight) intraperitoneally, and Group D, E, and F were given berberine in doses of 0.75 mg/kg, 1.25 mg/kg, and 2.5 mg/kg body weight respectively intraperitoneally for seven consecutive days, one hour after the potassium oxonate injection. On zero, first, third, and seventh day of the experiment, blood and urine samples were taken to estimate the serum and urinary uric acid levels. On days zero and 7, serum uric acid was measured by cardiac puncture, while on days 1 and 3, it was measured by the tail prick method. The uric acid was measured by an enzymatic colorimetric method and creatinine by the Jaffe method. Fractional excretion of urate was also calculated. Results Berberine lowered serum uric acid levels in rats with potassium oxonate-induced hyperuricemia with highly significant results (p-value <0.001) in all three dosages. Berberine increased the urinary uric acid level and the fractional excretion of urate in a time-dependent manner in all three dosages. This effect was maximally shown by low dose berberine with a highly significant result (p-value <0.001). Conclusion Berberine successfully decreased the serum uric acid level of hyperuricemic rats by increasing the urinary uric acid level and fractional excretion of urate.
背景 高尿酸血症的主要表现为痛风。如今有多种药物用于治疗痛风，但它们都伴有多种副作用。本研究观察了黄连素（来自中药）对氧嗪酸钾诱导的高尿酸血症大鼠血清和尿液尿酸水平的影响。
材料与方法 将36只成年健康雌性斯普拉格-道利大鼠随机分为6组，每组6只。为诱导高尿酸血症，除A组外，所有组在第1、3和7天腹腔注射氧嗪酸钾（250mg/kg）。A组为正常对照组，连续7天腹腔注射生理盐水。C组腹腔注射别嘌醇（5mg/kg体重），D组、E组和F组分别腹腔注射剂量为0.75mg/kg、1.25mg/kg和2.5mg/kg体重的黄连素，连续7天，在注射氧嗪酸钾1小时后给药。在实验的第0、1、3和7天，采集血液和尿液样本以评估血清和尿液尿酸水平。在第0天和第7天，通过心脏穿刺测量血清尿酸，而在第1天和第3天，通过尾尖采血法测量。尿酸采用酶比色法测定，肌酐采用Jaffe法测定。同时计算尿酸排泄分数。
结果 黄连素可降低氧嗪酸钾诱导的高尿酸血症大鼠的血清尿酸水平，所有三种剂量均有高度显著的结果（p值<0.001）。黄连素在所有三种剂量下均以时间依赖性方式增加尿液尿酸水平和尿酸排泄分数。低剂量黄连素的这种作用最为明显，结果具有高度显著性（p值<0.001）。
结论 黄连素通过增加尿液尿酸水平和尿酸排泄分数，成功降低了高尿酸血症大鼠的血清尿酸水平。
