Zhang Lili, Chen Huixiao, He Fengxi, Zhang Shiqian, Li Aihua, Zhang Aifeng, Zhang Anqi
Department of Obstetrics and Gynecology, Liaocheng People's Hospital, Liaocheng, China.
Shandong University, Jinan, China.
Front Oncol. 2021 Feb 25;11:581932. doi: 10.3389/fonc.2021.581932. eCollection 2021.
MicroRNAs (miRNAs) play important roles in tumorigenesis by controlling target gene expression. With opposing roles as a tumor suppressor or oncogene, microRNA-320a (miR-320a) was found to participate in tumor genesis and progression and also identified as a potentially useful marker in cancer diagnosis, treatment, and prognosis. To better understand the role of miR-320a in ovarian cancer, we investigated miR-320a expression in epithelial ovarian cancer (EOC) specimens as well as EOC cell lines and analyzed correlations between miR-320a expression and processes associated with EOC progression. The miR-320a level in EOC specimens was found to be associated with ovarian cancer progression and infiltration. Through and studies, we found that miR-320a significantly promoted the proliferation, migration, and invasion of EOC cells, and we identified RASSF8 as a target gene of miR-320a that was downregulated in EOC tissues and cell lines. downregulation of RASSF8 promoted the growth, migration, and invasion of EOC cells. Together these findings indicate that RASSF8 is a direct target of miR-320a, through which miR-320a promotes the progression of EOC.
微小RNA（miRNA）通过控制靶基因表达在肿瘤发生过程中发挥重要作用。作为一种肿瘤抑制因子或癌基因，微小RNA-320a（miR-320a）具有相反的作用，被发现参与肿瘤的发生和进展，并且还被确定为癌症诊断、治疗和预后中一种潜在有用的标志物。为了更好地了解miR-320a在卵巢癌中的作用，我们研究了miR-320a在上皮性卵巢癌（EOC）标本以及EOC细胞系中的表达情况，并分析了miR-320a表达与EOC进展相关过程之间的相关性。发现EOC标本中miR-320a水平与卵巢癌进展和浸润相关。通过[具体实验]和[具体实验]研究，我们发现miR-320a显著促进EOC细胞的增殖、迁移和侵袭，并且我们确定RASSF8是miR-320a的一个靶基因，其在EOC组织和细胞系中表达下调。RASSF8的下调促进了EOC细胞的生长、迁移和侵袭。这些发现共同表明RASSF8是miR-320a的直接靶标，miR-320a通过它促进EOC的进展。
