Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Food Biochem. 2021 Apr;45(4):e13693. doi: 10.1111/jfbc.13693. Epub 2021 Mar 14.
Obesity is a chronic inflammatory disease that represents a risk factor for number of diseases including diabetes, steatohepatitis, and cancer. Using safe natural compounds to ameliorate obesity and its related metabolic syndrome is an interesting spot for research. We investigated the regulatory role and the underlying mechanism of black seed oil (BSO) on high-fat diet (HFD)-induced obesity in rats. The study included two models: the first one aimed to study the prophylactic effect of BSO (BSO administration for 10 weeks along with HFD) while the second one aimed to study the treatment role of BSO (BSO administration starting from the 10th week for 4 weeks along with HFD). BSO significantly decreased insulin resistance and body weight characteristics in both models. It also normalized lipid profile. Moreover, histopathological examination confirmed these results as BSO significantly decreased adipocyte size and hepatic lipid deposition. Besides, BSO alleviated HFD-induced oxidative stress as indicated by significant increase in the total antioxidant capacity and significant decrease in liver malondialdehyde. Moreover, BSO decreased significantly liver gluconeogenic enzymes mRNA expressions (phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) and increased significantly heme oxygenase-1 (HO-1), nuclear factor erythroid-2-related factor-2 (Nrf2) and insulin receptor mRNA expressions. In conclusion, BSO represents a natural therapy that has the ability to prevent and treat HFD-induced obesity in rats that may be mediated through Nrf2/HO-1 pathway's activation and insulin receptor expression's increase. To our best knowledge, this study represents a novel study that investigates the regulatory role of BSO on Nrf2 pathway in preventing and treating HFD-induced obesity. PRACTICAL APPLICATIONS: Black seed oil is a natural available safe supplement, thus it can be used for prevention from obesity and even treatment of obesity and obesity related complications. Introducing of black seed oil in the treatment regimen of obese patients may be promising.
肥胖是一种慢性炎症性疾病，是多种疾病的危险因素，包括糖尿病、脂肪性肝炎和癌症。使用安全的天然化合物来改善肥胖及其相关的代谢综合征是一个有趣的研究领域。我们研究了黑种草油（BSO）对高脂肪饮食（HFD）诱导的肥胖大鼠的调节作用及其潜在机制。该研究包括两个模型：第一个模型旨在研究 BSO 的预防作用（BSO 给药 10 周，同时给予 HFD），第二个模型旨在研究 BSO 的治疗作用（BSO 给药 10 周开始，同时给予 HFD 4 周）。BSO 显著降低了两个模型中的胰岛素抵抗和体重特征。它还使血脂谱正常化。此外，组织病理学检查证实了这些结果，因为 BSO 显著减少了脂肪细胞大小和肝脂质沉积。此外，BSO 减轻了 HFD 诱导的氧化应激，表现为总抗氧化能力显著增加，肝丙二醛显著减少。此外，BSO 显著降低了肝糖异生酶 mRNA 表达（磷酸烯醇丙酮酸羧激酶和葡萄糖-6-磷酸酶），显著增加了血红素加氧酶-1（HO-1）、核因子红细胞 2 相关因子 2（Nrf2）和胰岛素受体 mRNA 表达。总之，BSO 代表一种天然疗法，具有预防和治疗大鼠 HFD 诱导肥胖的能力，其机制可能与 Nrf2/HO-1 通路的激活和胰岛素受体表达的增加有关。据我们所知，这项研究是一项关于 BSO 对 Nrf2 通路在预防和治疗 HFD 诱导肥胖中的调节作用的新研究。实际应用：黑种草油是一种天然、安全、可用的补充剂，因此可用于预防肥胖，甚至治疗肥胖及其相关并发症。在肥胖患者的治疗方案中引入黑种草油可能是有前途的。
