Department of Endocrinology, The Second Affiliated Hospital of Xi'an Jiaotong University.
Department of Pediatric, Xi'an NO.3 Hospital.
J Oleo Sci. 2022;71(5):709-720. doi: 10.5650/jos.ess21389.
Current time obesity is the major challenges globally and the incidence of the obesity has raised dramatically in current years. The obesity enhanced the various metabolic diseases such as diabetes, cardiac, cancer and steatohepatitis. Natural drug having the long history to ameliorate the obesity and its related metabolic disorder. In this experimental study, we scrutinized the anti-obesity effect of nimbolide against high fat diet (HFD) induced obesity in rats. Wistar rats were divided into 5 groups and each group contains 10 rats. The body weight, tissue weight was estimated at regular time. Carbohydrate, lipid, hepatic, inflammatory cytokines, antioxidant and inflammatory parameters were estimated. The mRNA expression was also estimated. Nimbolide treated groups significantly (p < 0.001) suppressed the body weight at dose dependent manner. Nimbolide significantly (p < 0.001) reduced the hepatic parameters and altered the antioxidant parameters such as thiobarbituric acid reactive substances (TBARS), glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx), superoxide mutase (SOD), glutathione S transferase (GST); decreased the level of inflammatory cytokines (IL-1β, IL-6, TNF-α). Nimbolide suppressed the mRNA expression of glucose-6-phosphatase HO-1 and nuclear factor erythroid-2 related factor-2 (Nrf2). Collectively, we can say that nimbolide having the capability to suppressed the HFD induced obesity via Nrf2/HO-1 pathway.
目前肥胖是全球面临的主要挑战，近年来肥胖的发病率急剧上升。肥胖增加了多种代谢性疾病，如糖尿病、心脏、癌症和脂肪性肝炎。天然药物具有改善肥胖及其相关代谢紊乱的悠久历史。在这项实验研究中，我们研究了印苦楝素（nimbolide）对高脂肪饮食（HFD）诱导肥胖大鼠的抗肥胖作用。Wistar 大鼠分为 5 组，每组 10 只。定期评估体重、组织重量。评估碳水化合物、脂质、肝、炎性细胞因子、抗氧化和炎症参数。还估计了 mRNA 表达。印苦楝素处理组以剂量依赖的方式显著（p<0.001）抑制体重增加。印苦楝素显著（p<0.001）降低肝参数，并改变抗氧化参数，如硫代巴比妥酸反应物质（TBARS）、谷胱甘肽（GSH）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、谷胱甘肽 S 转移酶（GST）；降低炎性细胞因子（IL-1β、IL-6、TNF-α）水平。印苦楝素抑制葡萄糖-6-磷酸酶 HO-1 和核因子红细胞 2 相关因子-2（Nrf2）的 mRNA 表达。总之，我们可以说印苦楝素通过 Nrf2/HO-1 通路具有抑制 HFD 诱导肥胖的能力。
