N-乙酰半胱氨酸对葡萄糖脱氢酶法葡萄糖计的干扰。
N-Acetylcysteine Interference with a Glucose Dehydrogenase Linked Glucose Meter.
机构信息
Laboratory Medicine, Division of Clinical Biochemistry, Saskatchewan Health Authority, Saskatoon, Saskatchewan, Canada.
出版信息
J Diabetes Sci Technol. 2022 Sep;16(5):1114-1119. doi: 10.1177/1932296821999416. Epub 2021 Mar 14.
BACKGROUND
Our objective was to determine the effect of therapeutic concentrations of N-acetylcysteine, following intravenous infusion, on the measurement of blood glucose using a Roche Diagnostics glucose dehydrogenase-linked glucose meter compared to hospital laboratory methods.
METHODS
N-acetylcysteine was added to aliquots of blood, with glucose promptly measured by the glucose meter, blood gas analyzer (glucose oxidase comparative method) and following centrifugation, plasma glucose measured with a hexokinase spectrophotometric comparative method. Glucose results were evaluated with linear regression and Bland Altman plots.
RESULTS
In the presence of NAC, at concentrations greater than 5 mg/dL (0.31 mmol/L), positively biased glucose meter results were compared to the clinical laboratory results. Multivariate linear regression revealed that NAC-mediated meter results are influenced by NAC and glucose concentrations.
CONCLUSIONS
The addition of therapeutic concentrations of NAC to blood produces statistically significant positive biases when measured with the glucose dehydrogenase linked glucose meter device.
背景
我们的目的是确定静脉输注治疗浓度的 N-乙酰半胱氨酸对罗氏诊断葡萄糖脱氢酶法葡萄糖计测量血糖的影响，与医院实验室方法进行比较。
方法
将 N-乙酰半胱氨酸加入血液样本中，使用葡萄糖计、血气分析仪（葡萄糖氧化酶比较法）即时测量血糖，然后离心，使用己糖激酶分光光度比较法测量血浆血糖。使用线性回归和 Bland-Altman 图评估血糖结果。
结果
在 NAC 浓度大于 5mg/dL（0.31mmol/L）的情况下，与临床实验室结果相比，NAC 会使葡萄糖计的结果产生正偏倚。多变量线性回归显示，NAC 介导的计数值受 NAC 和葡萄糖浓度的影响。
结论
当使用葡萄糖脱氢酶法葡萄糖计设备测量时，血液中添加治疗浓度的 NAC 会产生具有统计学意义的正偏倚。
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