Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia.
BIOCentre Ltd., Bioanalytical Laboratory II - Proteomics, Zagreb, Croatia.
Int J Environ Health Res. 2022 Jul;32(7):1581-1593. doi: 10.1080/09603123.2021.1898550. Epub 2021 Mar 9.
Bacterium is a leading cause of nosocomial infections. The occurrence of antibiotic-resistant isolates outside hospitals suggests that monitoring of this pathogen in environmental samples is needed. Survival of pandrug-resistant was followed on selective plates with and without carbapenems in water and soil. After a few days of starvation, lost the ability to be cultivated at 44°C on plates supplemented with carbapenems. Once cultivated on plates without carbapenems and/or at 36°C, could grow again at 44°C in the presence of carbapenems. Comparative proteomic analysis revealed that impaired membrane integrity and reduced function of efflux pumps due to elevated temperature combined with antibiotic exposure were the main reasons for this phenomenon. Loss of thermotolerance in the presence of antibiotics points to the need for temperature adjustment in long-term monitoring of in environmental samples, to avoid the underestimation of viable bacteria.
细菌是导致医院感染的主要原因。在医院外出现抗生素耐药分离株表明，需要对环境样本中的这种病原体进行监测。在含有和不含有碳青霉烯类抗生素的水和土壤选择性平板上，对泛耐药菌的存活情况进行了跟踪。经过几天的饥饿，在含有碳青霉烯类抗生素的平板上，44°C 时无法培养。一旦在不含碳青霉烯类抗生素和/或 36°C 的平板上培养，在碳青霉烯类抗生素存在的情况下，可再次在 44°C 下生长。比较蛋白质组学分析表明，由于温度升高和抗生素暴露导致的膜完整性受损和外排泵功能降低是这种现象的主要原因。在存在抗生素的情况下失去耐热性表明，在环境样本中对进行长期监测时需要调整温度，以避免低估活菌。
