Cosgrove L J, Vaughan H A, Tjandra J J, Thurlow P J, McKenzie I F
Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
Immunol Cell Biol. 1988 Feb;66 ( Pt 1):69-77. doi: 10.1038/icb.1988.9.
HLA (Class I) antigens are ubiquitous in their cellular distribution and, while their function in major histocompatibility complex (MHC)-restricted phenomena are clear, their function on other cells, such as platelets, is not so obvious. We now report that several anti-HLA monoclonal antibodies (including an anti-beta 2 microglobulin antibody) selectively affect platelet function in that three different anti-HLA monoclonal antibodies caused not only the aggregation of human platelets, but also caused the release of 14C-serotonin. In addition, the anti-HLA antibodies could selectively block the binding of several platelet agonists such as collagen, adrenalin, ADP, but not the binding of others such as thrombin and arachidonic acid. In blocking studies there also appeared to be an association between platelet glycoprotein IIb-IIIa and HLA Class I antigens. We propose that both heavy and light chains of Class I HLA antigens on platelets may be involved in platelet aggregation and release and suggest an additional role for HLA antigens on platelets.
HLA（I类）抗原在细胞分布上广泛存在，虽然它们在主要组织相容性复合体（MHC）限制现象中的功能已明确，但它们在其他细胞（如血小板）上的功能并不那么明显。我们现在报告，几种抗HLA单克隆抗体（包括一种抗β2微球蛋白抗体）选择性地影响血小板功能，因为三种不同的抗HLA单克隆抗体不仅导致人血小板聚集，还导致14C - 5羟色胺释放。此外，抗HLA抗体可以选择性地阻断几种血小板激动剂如胶原、肾上腺素、ADP的结合，但不能阻断其他激动剂如凝血酶和花生四烯酸的结合。在阻断研究中，血小板糖蛋白IIb - IIIa与HLA I类抗原之间似乎也存在关联。我们提出血小板上I类HLA抗原的重链和轻链可能都参与血小板聚集和释放，并提示HLA抗原在血小板上有额外作用。
