Kurata Y, Oshida M, Take H, Furubayashi T, Mizutani H, Tomiyama Y, Yonezawa T, Tarui S
Department of Blood Transfusion, Osaka University Hospital, Japan.
Vox Sang. 1990;59(2):106-11. doi: 10.1111/j.1423-0410.1990.tb05020.x.
The identification of antibodies to platelet-specific antigens is important for correctly diagnosing neonatal alloimmune thrombocytopenia, posttransfusion purpura and refractoriness due to platelet-specific antibodies. However, the serologic identification of these platelet-specific antibodies is complicated by the presence of anti-HLA antibodies. We examined and compared the diagnostic usefulness of acid-treated and chloroquine-treated platelets for the discrimination of platelet-specific antibodies from anti-HLA antibodies. The viability of acid-treated platelets is 83.4%, which is better than that of chloroquine-treated platelets (52.6%). The antigenicity of HLA class I antigens of acid-treated platelets was significantly reduced compared with that of PBS- or chloroquine-treated platelets. On the other hand, platelet surface glycoprotein Ib and glycoprotein IIb/IIIa, and platelet-specific antigens were stable following acid or chloroquine treatment. Chloroquine-treated platelets were not suitable targets for analysis by immunofluorescence flow cytometry because of nonspecific fluorescence derived from platelet damage. We conclude that acid-treated platelets are more suitable targets than chloroquine-treated platelets for screening for platelet-specific antibodies and also for analyses of the specificity of platelet-specific antibodies.
鉴定针对血小板特异性抗原的抗体对于正确诊断新生儿同种免疫性血小板减少症、输血后紫癜以及由血小板特异性抗体引起的难治性血小板减少症至关重要。然而，这些血小板特异性抗体的血清学鉴定因抗 HLA 抗体的存在而变得复杂。我们检测并比较了酸处理血小板和氯喹处理血小板在区分血小板特异性抗体与抗 HLA 抗体方面的诊断效用。酸处理血小板的活力为 83.4%，优于氯喹处理血小板（52.6%）。与 PBS 处理或氯喹处理的血小板相比，酸处理血小板的 HLA I 类抗原的抗原性显著降低。另一方面，酸或氯喹处理后，血小板表面糖蛋白 Ib 和糖蛋白 IIb/IIIa 以及血小板特异性抗原保持稳定。由于血小板损伤产生的非特异性荧光，氯喹处理的血小板不适用于免疫荧光流式细胞术分析。我们得出结论，对于筛选血小板特异性抗体以及分析血小板特异性抗体的特异性而言，酸处理血小板比氯喹处理血小板更适合作为检测对象。
