Opt Lett. 2021 Mar 15;46(6):1353-1356. doi: 10.1364/OL.419381.
Multispectral/hyperspectral fluorescence lifetime imaging microscopy (FLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field FLIM setup, based on a novel time-resolved 18×1 single-photon avalanche diode array detector working in a single-pixel camera scheme, which parallelizes the spectral detection, reducing measurement time. The proposed system, which implements a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework towards the design of FLIM setups.
多谱/高光谱荧光寿命成像显微镜（FLIM）是研究功能和结构生物过程的一种很有前途的工具。多维数据集提供的丰富信息内容通常与采集速度形成对比。在这项工作中，我们开发并实验证明了一种基于新型时间分辨 18×1 单光子雪崩二极管阵列探测器的宽场 FLIM 装置，该探测器在单像素相机方案中工作，实现了光谱检测的并行化，从而缩短了测量时间。所提出的系统采用基于压缩感知方案的单像素相机，代表了面向 FLIM 装置设计的最佳显微镜框架。
