Department of Parasitology, Pasteur Institute of Tunis, Tunis, Tunisia.
Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR 11IPT-06, Pasteur Institute of Tunis, University Tunis El-Manar, Tunis, Tunisia.
J Clin Lab Anal. 2021 May;35(5):e23749. doi: 10.1002/jcla.23749. Epub 2021 Mar 15.
An immunochromatography technology (ICT) rapid diagnostic test, the Toxoplasma ICT IgG-IgM , was recently developed. Our aim was to study its contribution to establish accurately the Toxoplasma immune status in Tunisian pregnant women using Western blot (WB) Toxo II IgG as a reference technique.
Thirty-nine sera were selected for the study from among 2,615 which were already tested by IgG and IgM ELISA. They displayed equivocal IgG titres (4.4-9 IU/ml) in absence of IgM (19 sera) or IgM anti-Toxoplasma antibodies in absence of IgG (titre <4.4 IU/ml) (20 sera). All these sera were additionally tested by WB Toxo II IgG .
Immunochromatography technology Sensitivity in the detection either of low IgG titres in absence of IgM or of specific anti-Toxoplasma IgM was 100%. Only one serum with equivocal IgG titre by ELISA and negative with Toxo II IgG test revealed positive in ICT. However, this serum showed a P30 band in WB analysis. On the other hand, three sera positive in ELISA IgM and negative in ELISA IgG revealed positive in ICT and negative in WB Toxo II IgG , the reference test.
Results confirm the high sensitivity of Toxoplasma ICT IgG-IgM in detecting both specific anti-Toxoplasma IgG and IgM, and highlight the usefulness of this rapid test as a first or second-line Toxoplasma serological test in pregnant women.
最近开发了一种免疫层析技术（ICT）快速诊断测试，即弓形虫 ICT IgG-IgM。我们的目的是使用 Western blot（WB）Toxo II IgG 作为参考技术，研究其在突尼斯孕妇中准确建立弓形虫免疫状态的贡献。
从已经通过 IgG 和 IgM ELISA 测试的 2615 个血清中选择了 39 个血清进行研究。这些血清的 IgG 滴度（4.4-9 IU/ml）为不确定（19 个血清），或者在没有 IgG（滴度<4.4 IU/ml）的情况下存在抗弓形虫 IgM（20 个血清）。所有这些血清均额外进行了 WB Toxo II IgG 测试。
免疫层析技术在检测 IgG 滴度低而无 IgM 或特异性抗弓形虫 IgM 方面的敏感性为 100%。只有一个 ELISA 检测 IgG 滴度不确定且 Toxo II IgG 检测阴性的血清在 ICT 中呈阳性。然而，该血清在 WB 分析中显示 P30 带。另一方面，三个 ELISA IgM 阳性而 ELISA IgG 阴性的血清在 ICT 中呈阳性，在 WB Toxo II IgG 检测中呈阴性，后者是参考检测。
结果证实了弓形虫 ICT IgG-IgM 在检测特异性抗弓形虫 IgG 和 IgM 方面的高敏感性，并强调了这种快速测试在孕妇中作为弓形虫血清学检测的一线或二线测试的有用性。
