Vaage J
Roswell Park Memorial Institute, Department of Experimental Pathology, Buffalo, NY 14263.
Int J Cancer. 1988 Jun 15;41(6):855-8. doi: 10.1002/ijc.2910410614.
This in vivo investigation determined the relative potentials of 7 C3H/He and C3Hf/He mammary tumors and their metastases to metastasize spontaneously from intramammary implants. The purpose of the studies was to examine the hypothesis that metastases derive from distinct sub-populations of cells in primary tumors which have specific inheritable characteristics that predispose the cells to form metastases. By serially transplanting, in parallel tests, tissue from autochthonous metastases and tissue from autochthonous primary tumors, the distinction of tumor-cell populations with different metastatic potentials was anticipated. However, the results of the experiments showed that primary tumors and metastases in autochthonous hosts had similar potentials for spontaneous metastasis. Moreover, the primary tumors and the metastases, transplanted as parallel lines through consecutive generations, maintained similar average metastasizing potentials. Changes in metastatic potential which occurred in 3 of the 7 tumors during serial passage appeared in nearly equal transplant generations in the parallel lines of the primary tumor and the metastases. The data suggest that the spontaneous metastases were not derived from a sub-population of cells with inheritable high metastasizing potential, but developed through stochastic events from the average tumor cells that entered the circulation.
这项体内研究确定了7种C3H/He和C3Hf/He乳腺肿瘤及其转移灶从乳腺内植入物自发转移的相对可能性。这些研究的目的是检验以下假设：转移灶源自原发性肿瘤中具有特定可遗传特征的不同细胞亚群，这些特征使细胞易于形成转移灶。通过在平行试验中对原位转移灶组织和原位原发性肿瘤组织进行连续移植，预期能够区分具有不同转移潜能的肿瘤细胞群体。然而，实验结果表明，原位宿主中的原发性肿瘤和转移灶具有相似的自发转移潜能。此外，作为平行线连续传代移植的原发性肿瘤和转移灶，其平均转移潜能保持相似。在7个肿瘤中的3个肿瘤连续传代过程中发生的转移潜能变化，在原发性肿瘤和转移灶的平行线中几乎出现在相同的移植代次。数据表明，自发转移并非源自具有可遗传高转移潜能的细胞亚群，而是通过随机事件从进入循环系统的普通肿瘤细胞发展而来。
