Department of Biology, Faculty of Arts and Sciences, Sakarya University, 54187, Serdivan, Sakarya, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Sakarya University, 54187, Serdivan, Sakarya, Turkey.
J Biol Inorg Chem. 2021 May;26(2-3):355-365. doi: 10.1007/s00775-021-01860-0. Epub 2021 Mar 15.
In this study, the chemical and algicidal properties of the newly synthesized compound (2) were evaluated and its algal oxidative effects were determined in Arthrospira platensis and Chlorella vulgaris. First, we have reported on the synthesis and characterization of highly water-soluble copper (II) phthalocyanine (2), containing sodium 2-mercaptoethanesulfonate (2) substituents at the peripheral positions. Some spectroscopic techniques were used to characterize the new synthesized compound (2). In terms of biological properties, C. vulgaris were more tolerance to compound (2) than A. platensis depending to growth parameters. When SOD (Superoxide dismutase) activity significantly increased at 0.25 ppb and 1.5 ppb concentrations in A. platensis cultures, it increased at 6 ppb concentration in C. vulgaris cultures. GR (Glutathione reductase) activity decreased at 1 ppb and 1.5 ppb concentrations while APX (Ascorbate peroxidase) activity did not show a significant change at any concentrations in A. platensis cultures. GR activity showed a significant increase at 6 ppb concentration, while APX activity increased at all concentrations compared to control in C. vulgaris cultures. MDA (malondialdehyde) and HO content decreased at 1 and 1.5 ppb concentrations but there were significant increases in the proline content at all concentrations compared to the control in A. platensis. MDA, HO and free proline contents showed a significant increase at 0.5 ppb concentration in C. vulgaris. In conclusion, compound (2) have algicidal effects, and also it causes to oxidative stress in these organisms.
在这项研究中，评估了新合成化合物 (2) 的化学和杀藻性质，并确定了其对蓝藻和普通小球藻的藻类氧化作用。首先，我们报道了高度水溶性铜（II）酞菁（2）的合成和表征，该化合物在其外围位置含有 2-巯基乙磺酸（2）取代基。使用一些光谱技术对新合成的化合物（2）进行了表征。就生物特性而言，与生长参数相比，C. vulgaris 比 A. platensis 对化合物（2）更具耐受性。当 SOD（超氧化物歧化酶）活性在 A. platensis 培养物中在 0.25 ppb 和 1.5 ppb 浓度下显著增加时，它在 C. vulgaris 培养物中在 6 ppb 浓度下增加。GR（谷胱甘肽还原酶）活性在 1 ppb 和 1.5 ppb 浓度下降低，而 APX（抗坏血酸过氧化物酶）活性在 A. platensis 培养物的任何浓度下均未显示出显著变化。GR 活性在 6 ppb 浓度下显示出显著增加，而 APX 活性在 C. vulgaris 培养物的所有浓度下均与对照相比均增加。与对照相比，在 A. platensis 中 MDA（丙二醛）和 HO 含量在 1 和 1.5 ppb 浓度下降低，但在所有浓度下脯氨酸含量均显著增加。在 C. vulgaris 中，MDA、HO 和游离脯氨酸含量在 0.5 ppb 浓度下显著增加。总之，化合物 (2) 具有杀藻作用，并且还会引起这些生物的氧化应激。
