Tunca Hatice, Çağatay Özpinar Sena, Tekbaba Ayşegül, Ongun Sevindik Tuğba, Doğru Ali, Günsel Armağan, Bilgiçli Ahmet T, Yaraşir M Nilüfer
Department of Biology, Faculty of Arts and Sciences, Sakarya University, Sakarya, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Sakarya University, Sakarya, Turkey.
Turk J Chem. 2021 Nov 22;46(2):367-377. doi: 10.3906/kim-2107-29. eCollection 2022.
In this study, a water-soluble metal-free phthalocyanine (SPC) containing sodium 2-mercaptoethanesulfonate substituents at the peripheral positions was used to investigate the algaecidal properties and oxidative effects on the growth of two microalgal species, and . Although OD at 560 nm and chlorophyll- content were decreased in during 7 days depending on dose and time, increases in both OD at 750 and chlorophyll- content at 8 ppb (parts per billion) concentration on the 7th day were observed in . However, total SOD (superoxide dismutase) and GR (glutathione reductase) enzyme activity of cultures did not display any alteration in all concentrations, SOD activity displayed an increase significantly at 2 ppb concentration, and GR activity showed increases at 1, 2, and 4 ppb concentrations in application. In application, APX (ascorbate peroxidase) activity decreased at 0.50 ppb, 1 ppb, and 1.5 ppb concentrations. In addition, application showed decreases at all concentrations. When MDA content increased at all concentrations, the HO content increased only at significatly 0.125 ppb concentration in cultures. Both MDA (malondialdehyde) and HO (hydrogen peroxide) content of cultures showed a statistically significant decrease at all concentrations compared to control. Free proline decreased at 0.25 ppb, 0.50 ppb, 1 ppb, and 1.5 ppb concentrations in application, and it decreased at all the concentrations of application. It concluded that this compound has inhibition effects on , but it supports growth in . Therefore, this synthesized phthalocyanine compound (SPC) should be consumed carefully, and the contamination to aquatic ecosystems should be prevented.
在本研究中，使用了一种在周边位置含有2-巯基乙磺酸钠取代基的水溶性无金属酞菁（SPC），以研究其对两种微藻物种 和 的杀藻特性及对其生长的氧化作用。尽管在7天内， 中的560 nm处的光密度（OD）和叶绿素含量根据剂量和时间而降低，但在第7天观察到 中8 ppb（十亿分之一）浓度下750 nm处的OD和叶绿素含量均增加。然而， 培养物的总超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GR）酶活性在所有浓度下均未显示任何变化，在 应用中，2 ppb浓度下SOD活性显著增加，1、2和4 ppb浓度下GR活性增加。在 应用中，抗坏血酸过氧化物酶（APX）活性在0.50 ppb、1 ppb和1.5 ppb浓度下降低。此外， 应用在所有浓度下均显示降低。当所有浓度下丙二醛（MDA）含量增加时， 培养物中仅在0.125 ppb浓度下过氧化氢（HO）含量显著增加。与对照相比， 培养物的MDA和HO含量在所有浓度下均显示出统计学上的显著降低。在 应用中，游离脯氨酸在0.25 ppb、0.50 ppb、1 ppb和1.5 ppb浓度下降低，在 应用的所有浓度下均降低。得出的结论是，该化合物对 有抑制作用，但对 有促进生长作用。因此，这种合成的酞菁化合物（SPC）应谨慎使用，并应防止对水生生态系统造成污染。
