Institute of Medical Biochemistry Leopoldo de Meis (IBqM), Federal University of Rio de Janeiro (UFRJ), 21941-590, Rio de Janeiro, RJ, Brazil.
Institute of Medical Biochemistry Leopoldo de Meis (IBqM), Federal University of Rio de Janeiro (UFRJ), 21941-590, Rio de Janeiro, RJ, Brazil; Multiuser Center for Biomolecular Innovation (CMIB) and Department of Physics, Institute of Biosciences, Letters and Exact Sciences (IBILCE), São Paulo State University (UNESP), 15054-000, São José do Rio Preto, SP, Brazil.
Curr Opin Virol. 2021 Apr;47:106-112. doi: 10.1016/j.coviro.2021.02.005. Epub 2021 Mar 12.
The Flaviviridae family comprises important human pathogens, including Dengue, Zika, West Nile, Yellow Fever and Japanese Encephalitis viruses. The viral genome, a positive-sense single-stranded RNA, is packaged by a single protein, the capsid protein, which is a small and highly basic protein that form intertwined homodimers in solution. Atomic-resolution structures of four flaviviruses capsid proteins were solved either in solution by nuclear magnetic resonance spectroscopy, or after protein crystallization by X-ray diffraction. Analyses of these structures revealed very particular properties, namely (i) the predominance of quaternary contacts maintaining the structure; (ii) a highly electropositive surface throughout the protein; and (iii) a flexible helix (α1). The goal of this review is to discuss the role of these features in protein structure-function relationship.
黄病毒科包括重要的人类病原体，如登革热、寨卡、西尼罗河、黄热病和日本脑炎病毒。病毒基因组是一条正链单链 RNA，由一种单一的蛋白质，衣壳蛋白，包装而成。衣壳蛋白是一种小而高度碱性的蛋白质，在溶液中形成相互交织的同源二聚体。通过核磁共振波谱法在溶液中或通过 X 射线衍射法在蛋白质结晶后，解析了四种黄病毒衣壳蛋白的原子分辨率结构。对这些结构的分析揭示了非常特殊的性质，即（i）主要存在维持结构的四级接触；（ii）整个蛋白质表面高度带正电荷；和（iii）一个灵活的螺旋（α1）。这篇综述的目的是讨论这些特征在蛋白质结构-功能关系中的作用。
