College of Food Science and Pharmaceutics, Xinjiang Agricultural University, Xinjiang, China; Tecon Biology Ltd, Xinjiang, China.
College of Animal Science, Xinjiang Agricultural University, Xinjiang, China.
Meat Sci. 2021 Jul;177:108353. doi: 10.1016/j.meatsci.2020.108353. Epub 2020 Oct 22.
We investigated ten pale, soft, and exudative (PSE), and ten normal meat samples from pig carcasses. The meat quality at 0, 5, 12, and 24 h post-mortem and the key enzyme activities at 0 and 24 h post-mortem were determined. We selected three PSE and three normal samples for proteomics analysis at 0 h and 24 h post-mortem. No remarkable differences in pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity were observed between samples at 0 h post-mortem; however, creatine kinase (CK) activity was significantly higher in PSE meat. Hexokinase (HK) activity in PSE samples was higher than that in normal samples at 24 h post-mortem. Bioinformatics analysis of the proteome showed that PSE was related to glycolysis, TCA cycle, oxidative phosphorylation, muscle tissue structure, signal transduction, and molecular chaperones. This research found that proteins such as troponin T slow skeletal muscle isoform X, GADPH, L-lactate dehydrogenase A chain, and gamma-enolase isoform X1 might be responsible for PSE.
我们研究了来自猪胴体的十个苍白、柔软和渗出（PSE）肉样和十个正常肉样。在宰后 0、5、12 和 24 h 测定了肉质和关键酶活性。在宰后 0 和 24 h 时，我们选择了三个 PSE 和三个正常肉样进行蛋白质组学分析。宰后 0 h 时，PK 和 LDH 活性在 PSE 和正常肉样之间没有显著差异；然而，CK 活性在 PSE 肉中显著更高。宰后 24 h 时，PSE 肉样中的 HK 活性高于正常肉样。蛋白质组学的生物信息学分析表明，PSE 与糖酵解、TCA 循环、氧化磷酸化、肌肉组织结构、信号转导和分子伴侣有关。本研究发现，肌钙蛋白 T 慢肌同工型 X、GAPDH、L-乳酸脱氢酶 A 链和 gamma-烯醇酶同工型 X1 等蛋白质可能是 PSE 的原因。
