Division of Surgical Oncology, Department of Surgery and the Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center, Dallas, Texas, USA.
Center for Pediatric Bone Biology and Translational Research, Scottish Rite for Children, Dallas, Texas, USA.
Lymphology. 2020;53(4):162-171.
Ectopic lymphatics form in bone and promote bone destruction in diseases such as Gorham-Stout disease, generalized lymphatic anomaly, and kaposiform lymphangiomatosis. However, the role lymphatics serve in normal bone development and repair is poorly understood. The objective of this study was to characterize bone development and fracture healing in mice that have a defect in the development of the lymphatic vasculature. We found that bones in wild-type adult mice and mouse embryos did not have lymphatics. We also found that bone development was normal in Vegfr3 (Chy/Chy) embryos. These mice do not have lymphatics and die shortly after birth. To determine whether lymphatics serve a role in postnatal bone development and fracture healing, we analyzed bones from Vegfr3 (wt/Chy) mice. These mice are viable and have fewer lymphatics than wild-type mice. We found that postnatal bone development and fracture healing was normal in Vegfr3 (wt/Chy) mice. Taken together, our results suggest that lymphatics do not play a major role in normal bone development or repair.
异位淋巴管在戈勒姆-斯图尔特病、全身性淋巴异常和卡波西样淋巴管瘤病等疾病中形成，并促进骨破坏。然而，淋巴管在正常骨发育和修复中的作用还知之甚少。本研究的目的是描述淋巴管发育缺陷小鼠的骨骼发育和骨折愈合情况。我们发现，野生型成年小鼠和胚胎小鼠的骨骼中没有淋巴管。我们还发现，Vegfr3（Chy/Chy）胚胎的骨骼发育正常。这些小鼠没有淋巴管，出生后不久就死亡。为了确定淋巴管是否在出生后骨骼发育和骨折愈合中发挥作用，我们分析了 Vegfr3（wt/Chy）小鼠的骨骼。这些小鼠具有活力，并且比野生型小鼠的淋巴管少。我们发现，Vegfr3（wt/Chy）小鼠的出生后骨骼发育和骨折愈合正常。总之，我们的结果表明，淋巴管在正常骨发育或修复中不起主要作用。
