ST8SIA6-AS1 通过 miR-338-3p/NONO 轴促进肝癌细胞的发展。
ST8SIA6-AS1 promotes the development of hepatocellular carcinoma cells through miR-338-3p/NONO Axis.
机构信息
Department of Gastroenterology, Qilu Hospital of Shandong University, Qingdao 266000, Shandong, China.
Department of General Surgery, Qilu Hospital of Shandong University, No.758 Hefei Road, Shibei District, Qingdao 266000, Shandong, China.
出版信息
Dig Liver Dis. 2021 Sep;53(9):1192-1200. doi: 10.1016/j.dld.2021.02.012. Epub 2021 Mar 12.
BACKGROUND
Increasing studies have shown a vital fact that long non-coding RNAs (lncRNAs) play a considerable regulatory role in hepatocellular carcinoma (HCC) progression. However, whether ST8 alpha-N-acetyl-neuraminide alpha-2, 8-sialyltransferase 6 antisense RNA 1 (ST8SIA6-AS1) affects the development of HCC is unclear.
METHODS
The target genes in HCC cell lines were quantified via utilzing quantitative real-time polymerase chain reaction (RT-qPCR) analysis and western blot. Effects of ST8SIA6-AS1 on proliferative, apoptosis and migratory ability of HCC cells were proved by a series of function experiments. The cellular distribution of ST8SIA6-AS1 was examined through fluorescent in situ hybridization (FISH) assay and subcellular fractionation experiments. RNA pulldown assay was implemented to explore the target of ST8SIA6-AS1. RNA Binding Protein Immunoprecipitation (RIP) and luciferase reporter assays were performed to identify the specific relationships between miR-338-3p and ST8SIA6-AS1/ non-POU domain containing octamer binding (NONO).
RESULTS
The expression of ST8SIA6-AS1 was apparently elevated in HCC cell. Silenced ST8SIA6-AS1 reduced proliferative, migratory and invasive ability of HCC cells. Moreover, ST8SIA6-AS1 targeted miR-338-3p to modulate the expression of NONO in HCC cells.
CONCLUSIONS
ST8SIA6-AS1 enhances the progression of HCC via miR-338-3p/NONO axis in vitro.
背景
越来越多的研究表明，长链非编码 RNA（lncRNA）在肝细胞癌（HCC）进展中发挥着重要的调节作用。然而，ST8 alpha-N-乙酰神经氨酸 alpha-2,8-唾液酸转移酶 6 反义 RNA 1（ST8SIA6-AS1）是否影响 HCC 的发展尚不清楚。
方法
通过定量实时聚合酶链反应（RT-qPCR）分析和 Western blot 定量 HCC 细胞系中的靶基因。通过一系列功能实验证实 ST8SIA6-AS1 对 HCC 细胞增殖、凋亡和迁移能力的影响。通过荧光原位杂交（FISH）检测和亚细胞分离实验检测 ST8SIA6-AS1 的细胞分布。通过 RNA 下拉实验探索 ST8SIA6-AS1 的靶基因。RNA 结合蛋白免疫沉淀（RIP）和荧光素酶报告基因检测实验用于鉴定 miR-338-3p 与 ST8SIA6-AS1/非 POUS 域包含八聚体结合（NONO）之间的特定关系。
结果
ST8SIA6-AS1 在 HCC 细胞中的表达明显升高。沉默 ST8SIA6-AS1 降低了 HCC 细胞的增殖、迁移和侵袭能力。此外，ST8SIA6-AS1 通过靶向 miR-338-3p 来调节 HCC 细胞中 NONO 的表达。
结论
ST8SIA6-AS1 通过体外 miR-338-3p/NONO 轴增强 HCC 的进展。
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