通过二维聚丙烯酰胺凝胶电泳对酵母线粒体tRNA的研究：同功受体种类的表征及对导入细胞质tRNA的探寻。
Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs.
作者信息
Martin R P, Schneller J M, Stahl A J, Dirheimer G
出版信息
Nucleic Acids Res. 1977 Oct;4(10):3497-510. doi: 10.1093/nar/4.10.3497.
By two-dimensional polyacrylamide gel electrophoresis, yeast mitochondrial tRNA is fractionated into 27 major species. All but 6 of them migrate distinctly from cytoplasmic tRNAs. Migration of mitochondrial DNA-coded mitochondrial tRNAs shows the occurence of only one cytoplasmic tRNA in mitochondria. Several mitochondrial tRNA spots are identified on the electrophoregrams, some of them show isoaccepting species (Val, Ser, Met, Leu). It is suggested that there are sufficient mitochondrial tRNA genes on yeast mitochondrial DNA to allow mitochondrial protein biosynthesis by the mitochondrial tRNAs alone. Guanosine + Cytidine content and rate base composition are reported for some individual species. Mitochondrial tRNAPhe lacks Ribothymidine.
通过二维聚丙烯酰胺凝胶电泳，酵母线粒体tRNA被分离为27种主要类型。其中除6种外，其余均与细胞质tRNA的迁移明显不同。线粒体DNA编码的线粒体tRNA的迁移表明线粒体中仅存在一种细胞质tRNA。在电泳图谱上鉴定出几个线粒体tRNA斑点，其中一些显示出同工受体类型（缬氨酸、丝氨酸、甲硫氨酸、亮氨酸）。有人提出，酵母线粒体DNA上有足够的线粒体tRNA基因，仅靠线粒体tRNA就能进行线粒体蛋白质生物合成。报告了一些个别类型的鸟苷+胞嘧啶含量和碱基组成比例。线粒体苯丙氨酸tRNA缺乏核糖胸腺嘧啶。
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