酿酒酵母线粒体tRNA的基因数量和碱基组成
Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.
作者信息
Schneller J M, Faye G, Kujawa C, Stahl A J
出版信息
Nucleic Acids Res. 1975 Jun;2(6):831-8. doi: 10.1093/nar/2.6.831.
Abstract
Increasing amounts of mitochondrial [32P] tRNA (4S fraction), were hybridized with mitochondrial DNA OF Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G+C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about 1T per sequence), U, hU are present in mitochondrial tRNA. Some sequence may begin with pG.
摘要
将越来越多的线粒体[32P] tRNA（4S级分）与酿酒酵母的线粒体DNA进行杂交。在饱和状态下，计算得出的4S线粒体RNA基因数量为20个。用过量的tRNA或过量的DNA获得的杂交体中洗脱出来的线粒体[32P] tRNA，经过碱性水解和色谱分析后，G+C含量分别为28%和35%。后一个值与在整个4S级分中发现的值相似。线粒体tRNA中存在奇数核苷酸T（每个序列约1个T）、U、hU。有些序列可能以pG开头。
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