Department of Chemistry, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Department of Chemistry, Graduate School of Science, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan.
Angew Chem Int Ed Engl. 2021 Jun 7;60(24):13603-13608. doi: 10.1002/anie.202102774. Epub 2021 May 6.
We demonstrate a fluid-fluid phase separation in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1-(2-hydroxybenzamide)-2-(2-hydroxy-3-formyl-5-hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (T ) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant-unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the T of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
我们使用 1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）膜中的金属配合物脂质 [Mn（L1）]（1；HL1=1-（2-羟基苯甲酰胺）-2-（2-羟基-3-甲酰基-5-十六烷氧基苯亚甲基氨基）乙）证明了液-液相分离。少量的 1 在 DMPC 中产生了两个分离的域，其脂质的相变温度（T）均低于原始 DMPC。DMPC/1 混合体的巨单层囊泡的变温荧光显微镜表明，可见的相分离在流体相中一直持续到 37°C，明显超过了 DMPC 的 T。这为金属配合物脂质在控制流体膜中脂质分布的应用提供了新的维度。
