Department of Chemistry, Fudan University, Shanghai 200433, China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology Shanghai Jiao Tong University, Shanghai 200240, China.
J Org Chem. 2021 Aug 20;86(16):11117-11124. doi: 10.1021/acs.joc.0c03056. Epub 2021 Mar 16.
Oligosaccharide natural products have diverse biological activities and represent a potentially important source for drug development. In this study, we focus on the glycosylation pathway in the biosynthesis of saccharomicin A (SA-A), an oligosaccharide antibiotic containing 17 sugar moieties. By extensive gene-knockout studies with comparative metabolic profile analysis, we established a complete pathway in assembling the heptadecasaccharide chain of SA-A, the longest saccharide chain found in natural products.
寡糖天然产物具有多样的生物活性，是药物开发的潜在重要来源。在这项研究中，我们专注于 saccharomicin A（SA-A）生物合成中的糖基化途径，SA-A 是一种含有 17 个糖基的寡糖抗生素。通过广泛的基因敲除研究和比较代谢谱分析，我们建立了一个完整的途径，用于组装 SA-A 的十七聚糖链，这是天然产物中发现的最长的糖链。
