Department of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of Turku, Turku, Finland.
Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
Eur J Oral Sci. 2021 Jun;129(3):e12783. doi: 10.1111/eos.12783. Epub 2021 Mar 16.
This aim of this study was to investigate the effects of three types of air-abrasion particles on dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis, both of which were cultured on sandblasted and acid-etched (SA) titanium discs. Out of 24 SA discs with biofilm, 18 were exposed to either air-abrasion using Bioglass 45S5 (45S5 BG; n = 6), novel zinc (Zn)-containing bioactive glass (Zn4 BG; n = 6), or inert glass (n = 6). The efficiency of biofilm removal was evaluated using scanning electron microscopy (SEM) imaging and culturing techniques. Air-abrasion using 45S5 BG or Zn4 BG demonstrated a significant decrease in the total number of viable bacteria compared to discs air-abraded with inert glass or intact biofilm without abrasion. Moreover, P. gingivalis could not be detected from SEM images nor culture plates after air-abrasion with 45S5 BG or Zn4 BG. The present study showed that air-abrasion with 45S5 or Zn4 bioactive glasses can successfully eradicate dual-biofilm of F. nucleatum and P. gingivalis from sandblasted and acid-etched titanium discs.
本研究旨在探讨三种气蚀颗粒对核梭杆菌和牙龈卟啉单胞菌双物种生物膜的影响，这两种细菌均在喷砂酸蚀（SA）钛片上培养。在 24 个带有生物膜的 SA 片中，有 18 个用 Bioglass 45S5（45S5BG；n=6）、新型含锌（Zn）生物活性玻璃（Zn4BG；n=6）或惰性玻璃（n=6）进行气蚀处理。使用扫描电子显微镜（SEM）成像和培养技术评估生物膜去除效率。与用惰性玻璃或未经磨损的完整生物膜进行气蚀处理的圆盘相比，使用 45S5BG 或 Zn4BG 进行气蚀处理显著降低了总活菌数。此外，在使用 45S5BG 或 Zn4BG 进行气蚀处理后，SEM 图像和培养平板上均无法检测到牙龈卟啉单胞菌。本研究表明，使用 45S5 或 Zn4 生物活性玻璃进行气蚀处理可以成功地从喷砂酸蚀钛片上清除核梭杆菌和牙龈卟啉单胞菌的双生物膜。
