Abushahba Faleh, Söderling Eva, Aalto-Setälä Laura, Hupa Leena, Närhi Timo O
Department of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of Turku, Finland.
Institute of Dentistry, University of Turku, Finland.
J Oral Implantol. 2019 Dec;45(6):444-450. doi: 10.1563/aaid-joi-D-18-00324. Epub 2019 Sep 19.
is able to form a high-affinity biofilm on material surfaces. has also been detected around infected implants. Bioactive glasses (BAGs) have been shown to possess antibacterial effects against and other microorganisms. This in vitro study was performed to investigate the influence of BAG air abrasion on biofilm on sandblasted and acid-etched titanium surfaces. Sandblasted and acid-etched commercially pure titanium discs were used as substrates for bacteria (n = 107). The discs were immersed in an solution and incubated for 21 hours to form an biofilm. Twenty colonized discs were subjected to air abrasion with Bioglass 45S5 (45S5 BAG), experimental zinc oxide containing BAG (Zn4 BAG), and inert glass. After the abrasion, the discs were incubated for 5 hours in an anaerobic chamber followed by an assessment of viable cells. Surface morphology was evaluation using scanning electron microscopy (n = 12). The thrombogenicity of the glass particle-abraded discs (n = 75) was evaluated spectrophotometrically using whole-blood clotting measurement at predetermined time points. Air abrasion with 45S5 and Zn4 BAG eradicated biofilm. Significantly fewer viable cells were found on discs abraded with the 45S5 or Zn4 BAGs compared with the inert glass ( < .001). No significant differences were found in thrombogenicity since blood clotting was achieved for all substrates at 40 minutes. Air abrasion with BAG particles is effective in the eradication of biofilm from sandblasted and acid-etched titanium surfaces. Zn4 and 45S5 BAGs had similar biofilm-eradicating effects, but Zn4 BAG could be more tissue friendly. In addition, the steady release of zinc ions from Zn4 may enhance bone regeneration around the titanium implant and may thus have the potential to be used in the treatment of peri-implantitis. The use of either BAGs did not enhance the speed of blood coagulation.
能够在材料表面形成高亲和力生物膜。在感染的植入物周围也检测到了它。生物活性玻璃（BAGs）已被证明对……和其他微生物具有抗菌作用。进行这项体外研究是为了调查BAG气磨对喷砂和酸蚀钛表面上……生物膜的影响。喷砂和酸蚀的商业纯钛圆盘用作细菌的底物（n = 107）。将圆盘浸入……溶液中并孵育21小时以形成……生物膜。二十个定植的圆盘用生物玻璃45S5（45S5 BAG）、含实验性氧化锌的BAG（Zn4 BAG）和惰性玻璃进行气磨。气磨后，将圆盘在厌氧箱中孵育5小时，然后评估存活的……细胞。使用扫描电子显微镜评估表面形态（n = 12）。在预定时间点使用全血凝血测量分光光度法评估玻璃颗粒磨损圆盘（n = 75）的血栓形成性。用45S5和Zn4 BAG进行气磨可根除……生物膜。与惰性玻璃相比，在用45S5或Zn4 BAG磨损的圆盘上发现的存活……细胞明显更少（P <.001）。由于所有底物在40分钟时都实现了血液凝固，因此在血栓形成性方面未发现显著差异。用BAG颗粒进行气磨可有效根除喷砂和酸蚀钛表面上的……生物膜。Zn4和45S5 BAG具有相似的生物膜根除效果，但Zn4 BAG可能对组织更友好。此外，Zn4中锌离子的稳定释放可能会增强钛植入物周围的骨再生，因此可能有潜力用于治疗种植体周围炎。使用任何一种BAG都不会提高血液凝固速度。
