Department of Chemistry, University of Central Florida, 4111 Libra Dr, PSB 255, Orlando, Fl 32816, USA.
Chem Commun (Camb). 2021 Apr 18;57(30):3672-3675. doi: 10.1039/d1cc00594d. Epub 2021 Mar 16.
Light-up DNA aptamers are promising label-free signal-transducers for biosensing applications due to their high chemical stability and low synthetic cost. Herein, we demonstrate that a dapoxyl DNA aptamer DAP-10-42 can be converted into a sensor generating a fluorescence signal at different wavelengths in the range of 500-660 nm depending on the dye that is present. This results from the discovered promiscuity of DAP-10-42 in binding fluorogenic dyes including arylmethane dyes. We have designed a split DAP-10-42 aptasensor for the detection of a katG gene fragment from Mycobacterium tuberculosis with a point mutation causing isoniazid resistance. Efficient interrogation of the gene fragment after nucleic acid sequence-based amplification (NASBA) is achieved directly in a protein-containing NASBA sample. This report lays a foundation for the application of the DAP-10-42 aptamer as a versatile sensing platform.
发夹型 DNA 适体由于其化学稳定性高和合成成本低，是很有前途的无标记信号转导物，可用于生物传感应用。本文中，我们证明了 dapoxyl DNA 适体 DAP-10-42 可以转化为传感器，根据存在的染料，在 500-660nm 的范围内产生不同波长的荧光信号。这是由于 DAP-10-42 与包括芳基甲烷染料在内的荧光染料结合的混杂性所致。我们设计了一种分裂的 DAP-10-42 适体传感器，用于检测结核分枝杆菌 katG 基因片段的点突变，该突变导致异烟肼耐药。在包含蛋白质的 NASBA 样品中直接进行基于核酸序列扩增 (NASBA) 后，对基因片段进行了有效的检测。该报告为 DAP-10-42 适体作为通用传感平台的应用奠定了基础。
