Brljak Nermina, Jin Ruitao, Walsh Tiffany R, Knecht Marc R
Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33146, USA.
Nanoscale. 2021 Mar 21;13(11):5670-5678. doi: 10.1039/d1nr00609f. Epub 2021 Mar 16.
The bio-recognition capabilities of materials-specific peptides offer a promising route to obtaining and organizing 2D nanosheet materials in aqueous media. Although significant advances have been made for graphene, little is currently understood regarding how to apply this strategy to hexagonal boron nitride (h-BN) due to a lack of knowledge regarding peptide/h-BN interactions. Here, one of the few peptide sequences known with affinity for h-BN, BP7, is the focus of mutation studies and bio-conjugation. A combination of experimental methods and modeling reveals the importance of Tyrosine in peptide/h-BN interactions. This residue is identified as the key anchoring species, which is then leveraged via bio-conjugation of BP7 to a fatty acid to create new interfacial properties. Specific placement of the fatty acid in the bio-conjugate results in dramatic manipulation of the surface-bound biotic overlayer to generate a highly viscoelastic interface. This viscoelasticity is a consequence of the fatty acid binding, which also down-modulates Tyrosine contact to h-BN, resulting in presentation of the extended peptide to solution. In this orientation, the biomolecule is available for subsequent bioconjugation, providing new pathways to programmable organization and conjugation of h-BN nanosheets in liquid water.
材料特异性肽的生物识别能力为在水性介质中获取和组织二维纳米片材料提供了一条很有前景的途径。尽管石墨烯已取得显著进展，但由于缺乏关于肽与六方氮化硼（h-BN）相互作用的知识，目前对于如何将该策略应用于h-BN了解甚少。在这里，少数已知对h-BN有亲和力的肽序列之一BP7是突变研究和生物共轭的重点。实验方法和建模相结合揭示了酪氨酸在肽与h-BN相互作用中的重要性。该残基被确定为关键的锚定物种，然后通过将BP7与脂肪酸进行生物共轭来利用这一特性，从而创造新的界面性质。脂肪酸在生物共轭物中的特定位置导致对表面结合的生物覆盖层进行显著调控，以产生高度粘弹性的界面。这种粘弹性是脂肪酸结合的结果，脂肪酸结合还会下调酪氨酸与h-BN的接触，从而使延伸的肽呈现于溶液中。在这种取向下，生物分子可用于后续的生物共轭，为在液态水中对h-BN纳米片进行可编程组织和共轭提供了新途径。
