Department of Laboratory Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea.
Medicine (Baltimore). 2021 Mar 19;100(11):e24852. doi: 10.1097/MD.0000000000024852.
We evaluated the capacity of the XN-350 instrument to analyze 3 different types of body fluid samples under "body fluid mode."The performance of XN-350 was evaluated in terms of precision, carryover, limit of blank, limit of detection, limit of quantification, and linearity. Cell enumeration and differential data produced by the XN-350 were compared to manual chamber counting results in 63 cerebrospinal fluid (CSF), 51 ascitic fluid, and 51 pleural fluid (PF) samples. Comparisons between XN-350 versus Cytospin data were also performed in PF samples.The precision, carry-over, limit of blank, and linearity of the XN-350 were acceptable. The limits of detection for white blood cells (WBCs) and red blood cells were 1.0/μL, and 1,000.0/μL, respectively; the corresponding limits of quantitation (LOQs) were 5.0/μL and 2,000.0/μL, respectively. The XN-350's cell enumeration and differential counting correlated well with those of manual chamber counting for all 3 sample types (except for differential counting in CSF samples), particularly parameters involving monocytes (r = 0.33) and mononuclear cells (MO- body fluid [BF]; r = 0.26), as well as total cell (TC-BF) enumeration (r = 0.50) and WBC-BF (r = 0.50) in PF samples. The MO-BF in CSF samples differed significantly from manual chamber counting results, but neither TC-BF nor WBC-BF in PF samples did. The XN-350 also showed good correlations with Cytospin analyses for differential counting of neutrophils, lymphocytes, and monocytes in PF samples. The differential counting of eosinophils via the XN-350 and Cytospin were not significantly correlated, but the difference between them was not significant.The XN-350 is an acceptable alternative to manual fluid analysis. Samples with low cellularity around the LOQ should be checked manually. Moreover, manual differential counting should be performed on CSF samples, particularity those with low cell numbers.
我们评估了 XN-350 仪器在“体液模式”下分析 3 种不同类型体液样本的能力。XN-350 的性能通过精密度、携带污染、空白限、检测限、定量限和线性来评估。XN-350 产生的细胞计数和分类数据与 63 份脑脊液 (CSF)、51 份腹水和 51 份胸腔积液 (PF) 样本的手动室计数结果进行了比较。还在 PF 样本中比较了 XN-350 与 Cytospin 数据之间的差异。XN-350 的精密度、携带污染、空白限和线性均符合要求。白细胞 (WBC) 和红细胞的检测限分别为 1.0/μL 和 1,000.0/μL；相应的定量限 (LOQ) 分别为 5.0/μL 和 2,000.0/μL。XN-350 的细胞计数和分类计数与所有 3 种样本类型的手动室计数结果相关性良好（CSF 样本的分类计数除外），特别是涉及单核细胞（r=0.33）和单核细胞（MO-体液 [BF]；r=0.26）以及总细胞（TC-BF）计数（r=0.50）和 PF 样本中的白细胞计数（r=0.50）。CSF 样本中的 MO-BF 与手动室计数结果有显著差异，但 PF 样本中的 TC-BF 或 WBC-BF 均无差异。XN-350 还与 PF 样本的 Cytospin 分析在中性粒细胞、淋巴细胞和单核细胞的分类计数方面具有良好的相关性。XN-350 与 Cytospin 分析在嗜酸性粒细胞的分类计数方面相关性不显著，但差异无统计学意义。XN-350 是一种替代手动液体分析的可行方法。接近定量限的低细胞数样本应手动检查。此外，CSF 样本应进行手动分类计数，特别是细胞数低的样本。
