验证新希森美康 XN-1000 体液模块对脑脊液和其他体液中血细胞计数的适用性。
Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids.
机构信息
Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
出版信息
Clin Chem Lab Med. 2012 Oct 1;50(10):1791-8. doi: 10.1515/cclm-2011-0927.
BACKGROUND
We evaluated the body fluid (BF) module on the new Sysmex XN-1000 for counting blood cells.
METHODS
One hundred and eighty-seven BF samples [73 cerebrospinal fluid (CSF), 48 continuous ambulatory peritoneal dialysis (CAPD), 46 ascites, and 20 pleural fluid] were used for method comparison between the XN-1000 and manual microscopy (Fuchs-Rosenthal chamber and stained cytospin slides) for counting red blood cells (RBCs) and white blood cells (WBCs) (differential).
RESULTS
Good agreement was found for counting WBCs (y=1.06x+0.09, n=67, R2=0.96) and mononuclear cells (MNs) (y=1.04x-0.01, n=40, R2=0.93) in CSF. However, the XN-1000 systematically counted more polymorphonuclear cells (PMNs) (y=1.48x+0.18, n=40, R2=0.99) compared to manual microscopy. Excellent correlation for RBCs >1×109/L (y=0.99x+116.56, n=26, R2=0.99) in CSF was found. For other fluids (CAPD, ascites and pleural fluid) excellent agreement was found for counting WBCs (y=1.06x+0.26, n=109, R2=0.98), MNs (y=1.06x-0.41, n=93, R2=0.96), PMNs (y=1.06x+0.81, n=93, R2=0.98) and RBCs (y=1.04x+110.04, n=43, R2=0.98). By using BF XN-check, the lower limit of quantitation (LLoQ) for WBC was defined at 5×106/L. Linearity was excellent for both the WBCs (R2=0.99) and RBCs (R2=0.99) and carry-over never exceeded 0.05%.
CONCLUSIONS
The BF module on the XN-1000 is a suitable tool for fast and accurate quantification of WBC (differential) and RBC counts in CSF and other BFs in a diagnostic setting.
背景
我们评估了新的 Sysmex XN-1000 上的体液（BF）模块，用于血细胞计数。
方法
使用 187 个 BF 样本[73 份脑脊液（CSF）、48 份连续流动腹膜透析（CAPD）、46 份腹水和 20 份胸腔积液]，比较 XN-1000 与手动显微镜（Fuchs-Rosenthal 室和染色细胞旋转涂片）在计数红细胞（RBC）和白细胞（WBC）（差异）方面的方法。
结果
在 CSF 中，XN-1000 对 WBC（y=1.06x+0.09，n=67，R2=0.96）和单核细胞（MNs）（y=1.04x-0.01，n=40，R2=0.93）的计数具有良好的一致性。然而，与手动显微镜相比，XN-1000 系统地计数了更多的多形核细胞（PMNs）（y=1.48x+0.18，n=40，R2=0.99）。在 CSF 中，对于 RBC>1×109/L（y=0.99x+116.56，n=26，R2=0.99），XN-1000 与手动显微镜的相关性也非常好。在 CSF 中，XN-1000 与手动显微镜的 WBC（y=1.06x+0.26，n=109，R2=0.98）、MNs（y=1.06x-0.41，n=93，R2=0.96）、PMN（y=1.06x+0.81，n=93，R2=0.98）和 RBC（y=1.04x+110.04，n=43，R2=0.98）计数也具有良好的一致性。使用 BF XN-check，WBC 的定量下限（LLOQ）定义为 5×106/L。WBC（R2=0.99）和 RBC（R2=0.99）的线性都非常好，且携带污染从未超过 0.05%。
结论
XN-1000 的 BF 模块是一种快速、准确地定量 CSF 和其他 BF 中 WBC（差异）和 RBC 计数的合适工具，适用于诊断环境。
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