Opt Express. 2021 Jan 18;29(2):552-563. doi: 10.1364/OE.408587.
An optical platform is presented for examining intrinsic contrast detection strategies when imaging retinal structure using ex vivo tissue. A custom microscope was developed that scans intact tissue and collects scattered light distribution at every image pixel, allowing digital masks to be applied after image collection. With this novel approach at measuring the spatial distribution of multiply scattered light, known and novel methods of detecting intrinsic cellular contrast can be explored, compared, and optimized for retinal structures of interest.
本文提出了一种光学平台，用于研究使用离体组织成像视网膜结构时内在对比度检测策略。开发了一种定制显微镜，可扫描完整组织并在每个图像像素处收集散射光分布，从而可以在图像采集后应用数字掩模。通过这种测量多重散射光空间分布的新方法，可以探索、比较和优化已知和新颖的内在细胞对比度检测方法，以用于感兴趣的视网膜结构。
