Tianjin Medical University, Teda International Cardiovascular Hospital, Tianjin, China.
Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular disease(Key Lab-TIC), Tianjin Institute of Cardiology (TIC), Department of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China.
J Renin Angiotensin Aldosterone Syst. 2021 Jan-Dec;22(1):14703203211003786. doi: 10.1177/14703203211003786.
To discuss the influence of PM2.5 on myocardial fibrosis and related mechanism.
PM2.5 particles were prepared into different concentrations of solution to drip into the mice's trachea twice each week. The mice were divided into five groups, Blank control group (C group), NS control group (J group), high dose group (G group, 10 mg/kg), medium dose group (Z group, 5 mg/kg), and 1ow dose group (D group, 2.5 mg/kg). After 6 weeks, the myocardial fibrosis was observed by HE and Masson staining. The expression of Ang II, ERK1/2, and TGF-β was examined by Western Blotting (WB) and Real time PCR (RT-PCR).
The higher dose PM2.5 was administrated, the worse the myocardial fibrosis was in PM2.5 groups. The expression of Ang II, ERK1/2, and TGF-β was increased in higher dose groups in protein and mRNA level.
探讨 PM2.5 对心肌纤维化的影响及相关机制。
将 PM2.5 颗粒制备成不同浓度的溶液，每周两次滴入小鼠气管。将小鼠分为五组：空白对照组（C 组）、NS 对照组（J 组）、高剂量组（G 组，10mg/kg）、中剂量组（Z 组，5mg/kg）和低剂量组（D 组，2.5mg/kg）。6 周后，通过 HE 和 Masson 染色观察心肌纤维化。通过 Western Blotting（WB）和 Real time PCR（RT-PCR）检测 Ang II、ERK1/2 和 TGF-β的表达。
PM2.5 剂量越高，PM2.5 组的心肌纤维化越严重。较高剂量组 Ang II、ERK1/2 和 TGF-β 的蛋白和 mRNA 水平表达增加。
