Tomkins P T, Carroll C V, Houghton J A
Department of Microbiology, University College, Galway, Ireland.
Hum Reprod. 1988 Apr;3(3):367-76. doi: 10.1093/oxfordjournals.humrep.a136710.
The prognostic value of the sperm penetration assay (SPA) using zona-free hamster ova has not been universally accepted. This is partly due to technical problems with the assay, resulting in 5-17% false negative results. The advantage of replacing albumin with defined synthetic polymers in the SPA culture media, increasing the calcium gradient and incorporating potential active tract agents and antioxidants, has been investigated. These studies have resulted in the development of a procedure that has produced no negative results among known fertile donors (n = 55). Application of this technique to a non-specific group of patients with suspected sub-fertility produced a different pattern of sperm penetration compared with the 'standard' SPA in 27% of cases. Improved sperm velocity, motile survival, enhanced hyaluronidase release and rate of acrosome induction were noted using the synthetic media.
使用去透明带仓鼠卵的精子穿透试验（SPA）的预后价值尚未得到普遍认可。部分原因是该试验存在技术问题，导致5%-17%的假阴性结果。人们研究了在SPA培养基中用特定合成聚合物替代白蛋白、增加钙梯度以及加入潜在活性物质和抗氧化剂的优势。这些研究促成了一种方法的开发，该方法在已知的可育供体（n = 55）中未产生阴性结果。将该技术应用于一组疑似亚生育的非特定患者，与“标准”SPA相比，27%的病例出现了不同的精子穿透模式。使用合成培养基时，观察到精子速度提高、活动存活能力增强、透明质酸酶释放增加以及顶体诱导率提高。
