Tomkins P T, Houghton J A
Department of Microbiology, University College, Galway, Ireland.
Fertil Steril. 1988 Aug;50(2):329-36.
Human spermatozoa are usually prepared for in vitro fertilization (IVF) by centrifugal washing and incubation in albumin based media. This is an inefficient technique contributing to poor IVF prospects, false-negative scores in the sperm penetration assay (SPA), and inadequate quantification in the sperm chromosome assay (SCA). High yields of fusiogenically functional sperm were rapidly and efficiently prepared by exposing sperm suspensions to an electrical pulse of 750 to 1500 V cm-1 for 2.5 msec. This process was calcium-dependent, and sperm incorporation into zona-free hamster eggs exhibited a linear relationship with Ca2+. At voltages greater than 1500 V cm-1, penetration declined. Sperm from subfertile men demonstrated significantly increased penetration after electropermeabilization. Sperm development was not hindered and in the SCA, yields of sperm metaphases approaching 100% were achievable.
人类精子通常通过离心洗涤并在基于白蛋白的培养基中孵育来制备用于体外受精（IVF）。这是一种效率低下的技术，导致IVF前景不佳、精子穿透试验（SPA）出现假阴性结果以及精子染色体试验（SCA）定量不足。通过将精子悬浮液暴露于750至1500 V cm-1的电脉冲2.5毫秒，可快速有效地制备出高产量的具有融合功能的精子。这个过程依赖于钙，并且精子与无透明带仓鼠卵的结合与Ca2+呈线性关系。在电压高于1500 V cm-1时，穿透率下降。不育男性的精子在电通透后显示出显著增加的穿透率。精子发育未受阻碍，在SCA中，可实现接近100%的精子中期产量。
