Grupo de investigación en Reumatología, Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña, Complejo Hospitalario Universitario de A Coruña, A Coruña, Spain.
Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, Norway.
Cytotherapy. 2021 May;23(5):399-410. doi: 10.1016/j.jcyt.2020.08.010. Epub 2021 Mar 13.
With the redefinition of osteoarthritis (OA) and the understanding that the joint behaves as an organ, OA is now considered a systemic illness with a low grade of chronic inflammation. Mitochondrial dysfunction is well documented in OA and has the capacity to alter chondrocyte and synoviocyte function. Transmitochondrial cybrids are suggested as a useful cellular model to study mitochondrial biology in vitro, as they carry different mitochondrial variants with the same nuclear background. The aim of this work was to study mitochondrial and metabolic function of cybrids with mitochondrial DNA from healthy (N) and OA donors. In this work, the authors demonstrate that cybrids from OA patients behave differently from cybrids from N donors in several mitochondrial parameters. Furthermore, OA cybrids behave similarly to OA chondrocytes. These results enhance our understanding of the role of mitochondria in the degeneration process of OA and present cybrids as a useful model to study OA pathogenesis.
随着骨关节炎 (OA) 的重新定义和对关节作为器官的理解，OA 现在被认为是一种全身性疾病，存在低度慢性炎症。OA 中线粒体功能障碍已有充分的记载，并有可能改变软骨细胞和滑膜细胞的功能。线粒体转移细胞杂种被认为是一种有用的细胞模型，可在体外研究线粒体生物学，因为它们具有相同核背景的不同线粒体变体。这项工作的目的是研究来自健康 (N) 和 OA 供体的线粒体 DNA 的细胞杂种的线粒体和代谢功能。在这项工作中，作者证明来自 OA 患者的细胞杂种在几个线粒体参数上与来自 N 供体的细胞杂种表现不同。此外，OA 细胞杂种的行为与 OA 软骨细胞相似。这些结果增强了我们对线粒体在 OA 退变过程中作用的理解，并提出细胞杂种是研究 OA 发病机制的有用模型。
