Department of Radiology, The Second Hospital of Dalian Medical University, Dalian 116027, People's Republic of China.
Department of Pediatrics, The Second Hospital of Dalian Medical University, Dalian 116027, People's Republic of China.
Magn Reson Imaging. 2021 Jun;79:59-65. doi: 10.1016/j.mri.2021.03.008. Epub 2021 Mar 13.
Hypoxic-ischemic brain damage (HIBD) is a critical disease in pediatric neurosurgery with high mortality rate and frequently leads to neurological sequelae. The role of bone marrow mesenchymal stem cells (BMSCs) in neuroprotection has been recognized. However, using the imaging methods to dynamically assess the neuroprotective effects of BMSCs is rarely reported. In this study, BMSCs were isolated, cultured and identified. Flow cytometry assay had shown the specific surface molecular markers of BMSCs, which indicated that the cultivated cells were purified BMSCs. The results demonstrated that CD29 and CD90 were highly expressed, whilst CD45 and CD11b were negatively expressed. Further, BMSCs were transplanted into Sprague Dawley (SD) rats established HIBD via three ways, including lateral ventricle (LV) injection, tail vein (TV) injection, and LV injection with magnetic guiding. Magnetic resonance imaging (MRI) was used to monitor and assess the treatment effect of super paramagnetic iron oxide (SPIO)-labeled BMSCs. The mean kurtosis (MK) values from diffusion kurtosis imaging (DKI) exhibited the significant differences. It was found that the MK value of HIBD group increased compared with that in Sham. At the meantime, the MK values of LV + HIBD, TV + HIBD and Magnetic+LV + HIBD groups decreased compared with that in HIBD group. Among these, the MK value reduced most significantly in Magnetic+LV + HIBD group. MRI illustrated that the treatment effect of Magnetic+LV + HIBD group was best. In addition, HE staining and TUNEL assay measured the pathological changes and apoptosis of brain tissues, which further verified the MRI results. All data suggest that magnetic guiding BMSCs, a targeted delivery way, is a new strategic theory for HIBD treatment. The DKI technology of MRI can dynamically evaluate the neuroprotective effects of transplanted BMSCs in HIBD.
缺氧缺血性脑损伤（HIBD）是小儿神经外科的一种严重疾病，死亡率高，常导致神经后遗症。骨髓间充质干细胞（BMSCs）在神经保护中的作用已得到认可。然而，使用影像学方法动态评估 BMSCs 的神经保护作用的报道很少。在这项研究中，我们分离、培养和鉴定了 BMSCs。流式细胞术检测显示 BMSCs 的特异性表面分子标志物，表明培养的细胞是纯化的 BMSCs。结果表明，CD29 和 CD90 高表达，而 CD45 和 CD11b 低表达。此外，我们通过三种方式将 BMSCs 移植到建立 HIBD 的 Sprague Dawley（SD）大鼠中，包括侧脑室（LV）注射、尾静脉（TV）注射和 LV 注射加磁引导。磁共振成像（MRI）用于监测和评估超顺磁性氧化铁（SPIO）标记的 BMSCs 的治疗效果。扩散峰度成像（DKI）的平均峰度（MK）值显示出显著差异。结果发现，与 Sham 组相比，HIBD 组的 MK 值增加。同时，LV+HIBD、TV+HIBD 和 Magnetic+LV+HIBD 组的 MK 值与 HIBD 组相比降低。其中，Magnetic+LV+HIBD 组的 MK 值降低最显著。MRI 表明，Magnetic+LV+HIBD 组的治疗效果最好。此外，HE 染色和 TUNEL 检测测量脑组织的病理变化和细胞凋亡，进一步验证了 MRI 结果。所有数据表明，磁引导 BMSCs 作为一种靶向输送方式，是 HIBD 治疗的新战略理论。MRI 的 DKI 技术可以动态评估移植 BMSCs 在 HIBD 中的神经保护作用。
