Department of Biobehavioral Health, Penn State University, University Park, PA, USA.
Department of Biobehavioral Health, Penn State University, University Park, PA, USA.
Neurotoxicol Teratol. 2021 May-Jun;85:106972. doi: 10.1016/j.ntt.2021.106972. Epub 2021 Mar 13.
Parental nicotine exposure can impact phenotypes in unexposed offspring. Our laboratory recently published data showing that nicotine reward and hippocampal gene expression involved in stress pathways were perturbed in F1 offspring of male C57BL/6J mice chronically exposed to nicotine. For the current study, we aimed to further test nicotine and stress-sensitivity phenotypes that may predict vulnerability to nicotine addiction in new cohorts of F1 offspring derived from nicotine-exposed males. We tested locomotor and body temperature sensitivity to acute nicotine administration, serum concentration of nicotine and nicotine metabolites after acute nicotine dosing, and serum corticosterone levels in male and female F1 offspring of nicotine- or saline-exposed males. Paternal nicotine exposure reduced sensitivity to nicotine-induced hypothermia in males, altered nicotine metabolite concentrations in males and females, and reduced serum basal corticosterone levels in females. These findings may point to reduced susceptibility to nicotine addiction-related phenotypes as a result of parental nicotine exposure.
父母尼古丁暴露会影响未暴露后代的表型。我们实验室最近发表的数据表明，慢性尼古丁暴露的雄性 C57BL/6J 小鼠的 F1 后代的尼古丁奖赏和参与应激途径的海马基因表达受到干扰。在当前的研究中，我们旨在进一步测试可能预测尼古丁成瘾易感性的新一批 F1 后代的尼古丁和应激敏感性表型，这些 F1 后代来自于暴露于尼古丁的雄性。我们测试了急性尼古丁给药后雄性和雌性 F1 后代的运动和体温对急性尼古丁给药的敏感性、血清中尼古丁和尼古丁代谢物的浓度，以及血清皮质酮水平。父代尼古丁暴露降低了雄性对尼古丁引起的体温降低的敏感性，改变了雄性和雌性的尼古丁代谢物浓度，并降低了雌性的基础血清皮质酮水平。这些发现可能表明，由于父母尼古丁暴露，与尼古丁成瘾相关的表型的易感性降低。
